STUDY OF THE MECHANISM RESPONSIBLE FOR THE ELECTIVE TOXICITY OF TUNGSTEN CARBIDE COBALT POWDER TOWARD MACROPHAGES
STUDY OF THE MECHANISM RESPONSIBLE FOR THE ELECTIVE TOXICITY OF TUNGSTEN CARBIDE COBALT POWDER TOWARD MACROPHAGES
复制标题
DOI：
10.1016/0378-4274(92)90275-o
复制
发表时间：
1992-04-01
影响因子：
3.5
通讯作者：
LAUWERYS, R
中科院分区：
文献类型：
--
作者：
LISON, D;LAUWERYS, R
我们先前已经证明碳化钨钴粉(WC-Co)在体外对小鼠巨噬细胞的毒性比纯金属钴颗粒更大，并且当金属以WC-Co混合物的形式存在时，细胞对钴的摄取被增强。本研究旨在探讨这种相互作用的可能机制(S)。我们发现WC的存在增加了钴在胞外环境中的增溶作用。然而，这一现象并不是解释WC-Co混合物毒性更大的关键因素，因为在没有WC的情况下，增加细胞外介质中溶解的钴量并不会导致毒性增加。此外，中毒剂量的WC-Co溶解的钴量本身不足以影响巨噬细胞的活力。只有当WC-Co混合物直接与细胞接触时，才能观察到毒性效应。WC-Co的选择性毒性也不能通过细胞外液中同时存在其他颗粒(WC)而刺激钴金属颗粒的吞噬作用来解释，因为乳胶球或酵母多糖颗粒对钴金属颗粒的吞噬作用并不能放大钴颗粒的毒性。这些结果表明，WC-Co混合物的毒性不仅仅是其钴组分的生物可利用性提高所致。这表明，硬质金属粉尘是一种特殊的有毒物质。
We have previously demonstrated that tungsten carbide-cobalt powder (WC-Co) is more toxic toward murine macrophages in vitro than pure cobalt metal particles and that the cellular uptake of cobalt is enhanced when the metal is present in the form of WC-Co mixture. The present study was undertaken to assess the possible mechanism(s) of this interaction. We found that solubilization of cobalt in the extracellular milieu was increased in the presence of WC. This phenomenon, however, is not the critical factor explaining the greater toxicity of the WC-Co mixture since increasing the amount of solubilized cobalt in the extracellular medium in the absence of WC did not result in increased toxicity. Moreover, the amount of cobalt solubilized from a toxic dose of WC-Co was insufficient to affect by itself macrophage viability. A toxic effect was only observed when the WC-Co mixture came directly in contact with the cells. The elective toxicity of WC-Co can also not be explained by stimulation of phagocytosis of cobalt metal particles due to the simultaneous presence of other particles (WC) in the extracellular fluid since stimulation of phagocytosis by latex beads or zymosan particles did not amplify the toxicity of cobalt metal particles. These results indicate that the toxicity of the WC-Co mixture does not simply result from an enhanced bioavailability of its cobalt component. This suggests that hard metal dust behaves as a specific toxic entity.