Surface Creasing Instability of Soft Polyacrylamide Cell Culture Substrates
Surface Creasing Instability of Soft Polyacrylamide Cell Culture Substrates
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DOI：
10.1016/j.bpj.2010.09.045
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发表时间：
2010-12-15
影响因子：
3.4
通讯作者：
Hayward, Ryan C.
中科院分区：
文献类型：
--
作者：
Saha, Krishanu;Kim, Jungwook;Hayward, Ryan C.
在机械软环境中理解和设计细胞行为的努力经常使用二维细胞培养基质，由刚性基质支撑的低弹性模量的薄水凝胶层组成，以促进培养，成像和分析。在这里，我们描述了最广泛使用的软细胞培养基质聚丙烯酰胺水凝胶凝胶表面的弹性折痕不稳定性是如何发生的，并表明干细胞对这些表面特征做出反应并改变其行为。在单体和交联剂浓度方面阐明了稳定区域和相应的可实现模量范围，为基础和应用细胞工程的光滑和折痕软细胞底物的合成提供了指导。
Efforts to understand and engineer cell behavior in mechanically soft environments frequently employ two-dimensional cell culture substrates consisting of thin hydrogel layers with low elastic modulus supported on rigid substrates to facilitate culturing, imaging, and analysis. Here we characterize how an elastic creasing instability of the gel surface may occur for the most widely used soft cell culture substrate, polyacrylamide hydrogels, and show that stem cells respond to and change their behavior due to these surface features. The regions of stability and corresponding achievable ranges of modulus are elucidated in terms of the monomer and cross-linker concentrations, providing guidance for the synthesis of both smooth and creased soft cell substrates for basic and applied cell engineering efforts.