Whole Mount In Situ Hybridization in Murine Tissues.
Whole Mount In Situ Hybridization in Murine Tissues.
复制标题
小鼠组织中的整体原位杂交。
DOI:
10.1007/978-1-0716-1028-2_22
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发表时间:
2021
期刊:
影响因子:
--
通讯作者:
Karner,CourtneyM
中科院分区:
文献类型:
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作者:
Sharma,Deepika;Hilton,MatthewJ;Karner,CourtneyM
Whole mount in situ hybridization is a sensitive method used to characterize the spatial and temporal expression of RNA transcripts throughout an entire tissue. This method is an excellent tool for studying gene expression during embryonic development. Here, we describe a procedure for digoxigenin labeled in situ hybridization on whole embryos.