Breast Cancer Reconstruction: Design Criteria for a Humanized Microphysiological System.
Breast Cancer Reconstruction: Design Criteria for a Humanized Microphysiological System.
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DOI：
10.1089/ten.tea.2020.0372
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发表时间：
2021-02
影响因子：
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通讯作者：
Trivia P Frazier;Christopher Williams;M. Henderson;T. Duplessis;Emma Rogers;Xiying Wu;K. Hamel;E. Martin;O. Mohiuddin;Shahensha Shaik;R. Devireddy;B. Rowan;Daniel Hayes;J. Gimble
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文献类型：
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作者：
Trivia P Frazier;Christopher Williams;M. Henderson;T. Duplessis;Emma Rogers;Xiying Wu;K. Hamel;E. Martin;O. Mohiuddin;Shahensha Shaik;R. Devireddy;B. Rowan;Daniel Hayes;J. Gimble
国际监管机构，如食品和药物管理局已要求科学界在不久的将来开发人源化微生理系统（MPS）作为动物模型的体外替代品。虽然乳腺癌研究界长期以来一直认识到三维（3D）生长动力学在其实验模型中的重要性，但仍存在障碍，阻止完全转化为人源化MPS用于药物发现和病理生理学研究。这种观点评估了人类组织来源的细胞和支架作为构建模块的“理想化”的乳腺癌MPS的生物工程设计原则的基础上，目前的状态。它考虑了脂肪组织作为支持乳腺癌上皮细胞的内皮细胞、淋巴造血细胞和基质细胞的潜在来源的效用。相对于目前的“金标准”材料Matrigel（一种鼠软骨肉瘤衍生的基底膜富集水凝胶），评价了来自脂肪组织、血液成分和合成生物材料的潜在MPS支架的相对优点。人源化乳腺癌MPS的优点和局限性在过程中和破坏性读出测定的背景下进行了讨论。
International regulatory agencies such as the Food and Drug Administration have mandated that the scientific community develop humanized microphysiological systems (MPS) as an in vitro alternative to animal models in the near future. While the breast cancer research community has long appreciated the importance of three-dimensional (3D) growth dynamics in their experimental models, there are remaining obstacles preventing a full conversion to humanized MPS for drug discovery and pathophysiological studies. This perspective evaluates the current status of human tissue-derived cells and scaffolds as building blocks for an "idealized" breast cancer MPS based on bioengineering design principles. It considers the utility of adipose tissue as a potential source of endothelial, lymphohematopoietic, and stromal cells for the support of breast cancer epithelial cells. The relative merits of potential MPS scaffolds derived from adipose tissue, blood components, and synthetic biomaterials is evaluated relative to the current "gold standard" material, Matrigel, a murine chondrosarcoma-derived basement membrane-enriched hydrogel. The advantages and limitations of a humanized breast cancer MPS are discussed in the context of in-process and destructive read-out assays.