Identification of a subset of murine natural killer cells that mediates rejection of Hh-1d but not Hh-1b bone marrow grafts.
Identification of a subset of murine natural killer cells that mediates rejection of Hh-1d but not Hh-1b bone marrow grafts.
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DOI：
10.1084/jem.170.1.191
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发表时间：
1989-07-01
期刊：
影响因子：
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通讯作者：
Bennett M
中科院分区：
文献类型：
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作者：
Sentman CL;Hackett J Jr;Kumar V;Bennett M
NK 细胞在体外和体内表现出许多免疫功能，包括裂解肿瘤或病毒感染的细胞以及排斥骨髓同种异体移植物。然而，目前尚不清楚是否所有 NK 细胞都能介导这些不同的功能，或者 NK 细胞是否存在于功能不同的亚群中。我们开发了一种新的 NK 特异性 mAb SW5E6，它可以与大约 50% 的小鼠 NK 细胞结合。 5E6 抗原可识别 NK 细胞的独特且稳定的子集，并在大约一半的新鲜或 rIL-2 激活的小鼠 NK 细胞上表达。 5E6+和5E6-NK细胞都能够在体外和体内裂解YAC-1肿瘤细胞。通过用 SW5E6 治疗动物，我们证明 5E6+ 子集对于 H-2d/Hh-1d 骨髓细胞的排斥是必需的，但对于 H-2b/Hh-1b 骨髓细胞则不然。因此，NK 细胞在体内作为功能上可分离的亚群存在。
NK cells demonstrate many immune functions both in vitro and in vivo, including the lysis of tumor or virus-infected cells and the rejection of bone marrow allografts. However it remains unclear whether or not all NK cells can mediate these various functions or if NK cells exist in functionally distinct subsets. We have developed a new NK-specific mAb, SW5E6, which binds to approximately 50% of murine NK cells. The 5E6 antigen identifies a distinct and stable subset of NK cells and is expressed on about one-half of fresh or rIL-2-activated murine NK cells. Both 5E6+ and 5E6- NK cells are capable of lysing YAC-1 tumor cells in vitro and in vivo. By treating animals with SW5E6, we demonstrate that the 5E6+ subset is necessary for the rejection of H- 2d/Hh-1d but not H-2b/Hh-1b bone marrow cells. Thus NK cells exist as functionally separable subsets in vivo.