DEVELOPMENT OF SYMPATHETIC NEURONS IN COMPARTMENTALIZED CULTURES .1. LOCAL-CONTROL OF NEURITE GROWTH BY NERVE GROWTH-FACTOR
DEVELOPMENT OF SYMPATHETIC NEURONS IN COMPARTMENTALIZED CULTURES .1. LOCAL-CONTROL OF NEURITE GROWTH BY NERVE GROWTH-FACTOR
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DOI：
10.1016/0012-1606(82)90232-9
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发表时间：
1982-01-01
影响因子：
2.7
通讯作者：
CAMPENOT, RB
中科院分区：
文献类型：
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作者：
CAMPENOT, RB
将新生大鼠颈上神经节的交感神经元接种于3个隔室的中央隔室，观察局部7S神经生长因子(NGF)浓度对侧室内神经突起伸展和密度的影响。从侧室的培养液中去掉大鼠血清和抗坏血酸有利于观察那里的轴突生长，而这些成分是通过中央隔室提供给神经元的。在此条件下，7S NGF浓度在0.01-10微克/毫升范围内，轴突延长率几乎没有差异(apprx。1毫米/天)。观察到对纤维密度的显著影响，在给予1-10µg NGF/ml的侧室中产生的密度比在给予1toreq的相同培养的相对侧室中产生的密度高。0.1微克/毫升。无论包含细胞体的中心隔室是否提供1微克NGF/毫升，都能观察到这一点。在缺乏NGF的中央隔室，轴突生长不明显，而暴露于NGF的轴突在侧室神经切断后再生。NGF对生长的促进主要(可能完全)局限于直接暴露于细胞外NGF的远端轴突区域，而在没有细胞外NGF的情况下，更近端的轴突区域(可能通过逆行运输含有NGF)对生长几乎没有促进作用。神经突起局部环境中的NGF浓度对其局部生长起着重要的控制作用，并有力地支持了组织中NGF水平调节体内交感神经支配的假说。
Sympathetic neurons from the superior cervical ganglia of newborn rats were plated in the center compartments of 3 compartment dishes and the effects of the local concentration of 7S nerve growth factor (NGF) on neurite extension and density in the side compartments were observed. Omission of rat serum and ascorbate from the culture medium in the side compartments proved favorable for the observation of neurite growth there, while these ingredients were supplied to the neurons via the center compartments. Under these conditions, 7S NGF concentrations ranging 0.01-10 .mu.g/ml produced little difference in the rate of neurite extension (.apprx. 1 mm/day). A substantial influence on fiber density was observed, with higher densities produced in side compartments given 1-10 .mu.g NGF/ml than in opposite side compartments of the same cultures given .ltoreq. 0.1 .mu.g/ml. This was observed irrespective of whether the center compartment containing the cell bodies was provided with 1 .mu.g NGF/ml or no NGF. Neurite growth was not apparent in NGF-deprived center compartments, while neurites exposed to NGF regenerated after neuritotomy in the side compartments. The promotion of growth by NGF was largely (perhaps entirely) confined to the distal neurite regions directly exposed to extracellular NGF, while the growth of more proximal neurite regions (which may be presumed to contain NGF en route to the soma via retrograde transport) was promoted little or not at all in the absence of extracellular NGF. The NGF concentration in the local environment of the neurites exerts important control over their local growth and strongly supports the hypothesis that tissue NGF levels regulate sympathetic innervation in vivo.