Uptake efficiency of surface modified gold nanoparticles does not correlate with functional changes and cytokine secretion in human dendritic cells in vitro
Uptake efficiency of surface modified gold nanoparticles does not correlate with functional changes and cytokine secretion in human dendritic cells in vitro
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DOI：
10.1016/j.nano.2014.11.004
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发表时间：
2015-04-01
影响因子：
5.4
通讯作者：
Rothen-Rutishauser, Barbara
中科院分区：
文献类型：
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作者：
Fytianos, Kleanthis;Rodriguez-Lorenzo, Laura;Rothen-Rutishauser, Barbara
用于免疫调节的工程纳米颗粒（NP）需要彻底了解它们与细胞的相互作用。金纳米颗粒（AuNPs）与聚乙二醇（PEG），聚乙烯醇（PVA）或两者的混合物与正或负表面电荷包被，以研究单核细胞衍生的树突状细胞（MDDC）的摄取和细胞反应。电感耦合等离子体发射光谱法和透射电子显微镜用于确认MDDC内Au的存在。分析细胞活力、（促）炎症反应、MDDC表型、活化标志物、抗原摄取和加工。仅在最高浓度的PVA-NH 2 AuNP中观察到细胞死亡。MDDC内化AuNP，然而，表面修饰影响摄取。尽管观察到PEG-COOH AuNP的摄取有限，但诱导了显著的肿瘤坏死因子-α释放。相比之下，（PEG + PVA）-NH 2和PVA-NH 2金纳米颗粒被内化到更高的程度，并引起白细胞介素-1 β分泌。没有一个AuNP引起MDDC表型、活化或免疫学特性的变化。(C)2015 Elsevier Inc. All rights reserved.
Engineering nanoparticles (NPs) for immune modulation require a thorough understanding of their interaction(s) with cells. Gold NPs (AuNPs) were coated with polyethylene glycol (PEG), polyvinyl alcohol (PVA) or a mixture of both with either positive or negative surface charge to investigate uptake and cell response in monocyte-derived dendritic cells (MDDCs). Inductively coupled plasma optical emission spectrometry and transmission electron microscopy were used to confirm the presence of Au inside MDDCs. Cell viability, (pro-) inflammatory responses, MDDC phenotype, activation markers, antigen uptake and processing were analyzed. Cell death was only observed for PVA-NH2 AuNPs at the highest concentration. MDDCs internalize AuNPs, however, surface modification influenced uptake. Though limited uptake was observed for PEG-COOH AuNPs, a significant tumor necrosis factor-alpha release was induced. In contrast, (PEG + PVA)-NH2 and PVA-NH2 AuNPs were internalized to a higher extent and caused interleukin-1beta secretion. None of the AuNPs caused changes in MDDC phenotype, activation or immunological properties. (C) 2015 Elsevier Inc. All rights reserved.