Choroidal thickness predicts ocular growth in normal chicks but not in eyes with experimentally altered growth.
Choroidal thickness predicts ocular growth in normal chicks but not in eyes with experimentally altered growth.
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DOI：
10.1111/cxo.12317
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发表时间：
2015-11
影响因子：
1.9
通讯作者：
Totonelly K
中科院分区：
文献类型：
--
作者：
Nickla DL;Totonelly K
在刚孵化的小鸡中，脉络膜的厚度变化很大。据推测，厚度本身或早期生活中发生的变化可能在调节眼部生长中起因果作用。我们通过在几个改变眼部生长的实验条件下测量眼部尺寸来测试这个概念。12 ~ 14日龄雏鸡配戴单目镜镜片或扩散器（+10 D, n = 23; - 10 D, n = 16；扩散器，n = 16） 4 ~ 5天。其他未治疗的眼睛作为对照。另一组完全未经治疗的8天大的鸟类也进行了测试（n = 12）。我们测试了两种已知能改变眼部生长的药物。每天注射多巴胺能激动剂喹匹罗，连续5天进入佩戴阴性镜片的眼睛（n = 47）。正常眼注射毒蕈碱激动剂氧tremorine一次（n = 27）。在实验开始和结束时，使用高频a型超声扫描测量所有眼睛。所有分析均采用Spearman相关系数。脉络膜厚度预测正常眼睛的生长速度：脉络膜薄的眼睛比脉络膜厚的眼睛生长得快（p = 0.0001）。初始厚度与厚度变化呈负相关（p = 0.0353）。相比之下，戴隐形眼镜或扩散眼镜的眼睛在初始厚度和生长速率之间没有相关性。对于注射了减慢生长速度的晶状体眼，初始脉络膜厚度预测了随后的生长速度（p = 0.0126），与正常眼相似。而刺激生长的氧tremorine则不是这样。在实验改变生长的眼睛中，脉络膜厚度与随后的生长速率之间的关联缺失意味着脉络膜反应与眼睛生长的视觉调节分离。注射喹匹罗后，初始厚度与眼部生长呈负相关，提示较厚的脉络膜有潜在的治疗益处。
In hatchling chicks, the thickness of the choroid is quite variable. It has been postulated that thickness per se or the changes occurring during early life might play a causal role in the regulation of ocular growth. We tested this notion by measuring ocular dimensions in several experimental conditions that alter ocular growth and in the fellow eyes. Chicks aged 12 to 14 days wore monocular lenses or diffusers (+10 D, n = 23; −10 D, n = 16; diffusers, n = 16) for four to five days. Fellow untreated eyes served as controls. A separate group of completely untreated birds aged eight days were also tested (n = 12). We tested two drugs known to alter ocular growth. The dopaminergic agonist quinpirole was injected daily for five days into eyes wearing negative lenses (n = 47). The muscarinic agonist oxotremorine was injected one time into normal eyes (n = 27). All eyes were measured using high-frequency A-scan ultrasonography at the start and end of the experiment. Spearman’s correlation coefficient was used in all analyses. Choroidal thickness predicted ocular growth rates in normal eyes: eyes with thinner choroids grew faster than those with thicker choroids (p = 0.0001). Furthermore, there was a negative correlation between initial thickness and the change in thickness (p = 0.0353). By contrast, eyes wearing lenses or diffusers did not show a correlation between initial thickness and growth rate. For lens-wearing eyes injected with quinpirole, which slowed growth, initial choroidal thickness predicted subsequent growth rate (p = 0.0126), similar to normal eyes. This was not so for oxotremorine, which stimulated growth. The loss of the association between choroidal thickness and subsequent growth rate in eyes with experimentally altered growth implies an uncoupling of the choroidal response from the visual regulation of ocular growth. The negative correlation between initial thickness and ocular growth in eyes injected with quinpirole suggests potential therapeutic benefits to thicker choroids.