CYTOTOXICITY OF 109 CHEMICALS TO GOLDFISH GFS CELLS AND RELATIONSHIPS WITH 1-OCTANOL WATER PARTITION-COEFFICIENTS
CYTOTOXICITY OF 109 CHEMICALS TO GOLDFISH GFS CELLS AND RELATIONSHIPS WITH 1-OCTANOL WATER PARTITION-COEFFICIENTS
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DOI：
10.1016/0045-6535(93)90374-e
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发表时间：
1993-03-01
期刊：
影响因子：
8.8
通讯作者：
KOIKE, S
中科院分区：
文献类型：
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作者：
SAITO, H;KOYASU, J;KOIKE, S
将醇类、芳香剂、酚类、农药等109种化学物质对金鱼鳞GFS细胞的中点细胞毒性（NR50值）与鱼急性毒性及1-辛醇/水分配系数（Pow）进行比较。 NR50 值与黑头呆鱼 (n = 31, r = 0.958, s = 0.480, F = 325) 和孔雀鱼 (n = 29, r = 0.957, s = 0.482, F = 294) 的可用急性毒性数据显着相关。除了 NR50 的 8 种化合物外，Pow 在简单线性回归分析中对所有化合物都给出了良好的相关性。由于它们在培养基中的溶解度低，无法获得数值。 Log 1/NR50 (mM) = (0.87 +/- 0.08) LogPow- (2.17 +/- 0.24) (n = 101, r = 0.914, s = 0.587, F = 505) 对于 29 种麻醉类型的化合物，回归分析显示 NR50 值与 Pow 之间存在高度相关性，并且似乎给出了基线细胞毒性。 Log 1/NR50 (mM) = (0.77 +/- 0.06) LogPow- (2.48 +/- 0.12) (n = 29, r = 0.984, s = 0.252, F = 817)此外，还讨论了某些类型化合物的 NR50 和 Pow 之间的回归分析。这些结果表明 NR 测定可用于预测对鱼类的急性毒性和定量研究结构-活性关系（QSAR）。
The midpoint cytotoxicity (NR50 values) of 109 chemicals including alcohols, aromatics, phenols and pesticides to goldfish scale GFS cells were compared with the fish acute toxicities and with the 1 - octanol/water partition coefficient (Pow). The NR50 values were significantly correlated to the available acute toxicity data for fathead minnow (n = 31, r = 0.958, s = 0.480, F = 325) and guppy (n = 29, r = 0.957, s = 0.482, F = 294).The Pow gave a good correlation in simple linear regression analysis for all compounds excluding 8 compounds that the NR50 values could not be obtained for their low solubility in culture medium. Log 1/NR50 (mM) = (0.87 +/- 0.08) LogPow- (2.17 +/- 0.24) (n = 101, r = 0.914, s = 0.587, F = 505)For 29 narcotic type of compounds, the regression analysis revealed a high correlation between the NR50 values and Pow, and appeared to give a baseline cytotoxicity. Log 1/NR50 (mM) = (0.77 +/- 0.06) LogPow- (2.48 +/- 0.12) (n = 29, r = 0.984, s = 0.252, F = 817)And also, the regression analyses between NR50 and Pow for some types of compounds were discussed.These results suggest that NR assay is useful for prediction of acute toxicity to fish and studies of quantitative structure-activity relationships (QSARs).