Dielectrophoretic manipulation and characterization of herpes simplex virus-1 capsids
Dielectrophoretic manipulation and characterization of herpes simplex virus-1 capsids
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DOI：
10.1007/s002490100144
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发表时间：
2001-08-01
影响因子：
2
通讯作者：
Rixon, FJ
中科院分区：
文献类型：
--
作者：
Hughes, MP;Morgan, H;Rixon, FJ
在添加或不添加甘露醇的 KCl 溶液的一系列电导率下，测量了 1 型单纯疱疹病毒衣壳的介电泳行为。通过测量对应于零介电泳力的频率来记录衣壳的介电泳响应。使用多壳模型分析数据，并估计颗粒的介电常数和电导率。衣壳被建模为悬浮介质穿过的多孔蛋白质壳、含有与外部平衡的悬浮介质的内室以及蛋白质的中央核心（支架）。悬浮在不含甘露醇的 KCl 中的衣壳与悬浮在含有甘露醇的 KCl 中的衣壳表现出不同的行为。
The dielectrophoretic behaviour of the capsids of herpes simplex virus type-1 has been measured over a range of conductivities of KCl solutions, with and without the addition of mannitol. The dielectrophoretic response of the capsids was recorded by measuring the frequency corresponding to zero dielectrophoretic force. The data were analysed using a multi-shelled model, and the permittivity and conductivity of the particles estimated. The capsid was modelled as a porous protein shell through which suspending medium passes, an inner chamber containing suspending medium in equilibrium with the outside, and a central core of protein (the scaffold). Capsids suspended in KCl without mannitol exhibited a different behaviour to those suspended in KCl with mannitol.