Relationship between periodontal disease status and combination of biochemical assays of gingival crevicular fluid
Relationship between periodontal disease status and combination of biochemical assays of gingival crevicular fluid
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DOI：
10.1111/j.1600-0765.2005.00807.x
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发表时间：
2005-08-01
影响因子：
3.5
通讯作者：
Shizukuishi, S
中科院分区：
文献类型：
--
作者：
Hanioka, T;Matsuse, R;Shizukuishi, S
背景资料：目前，没有生化检测涉及龈沟液是利用常规作为牙周病的筛查test.Objective：本研究的目的是评估潜在的龈沟液检测作为一种筛查methodology.Methods：受试者的人口是由27名志愿者。9名参与者被归类为“牙周破坏受试者”（SPD），表现出至少一个部位的牙周袋深度和附着丧失> 3.5 mm，而其余个体被归类为“牙周破坏最小受试者”（SMD）。通过标准化方法从固定部位收集龈沟液。在商业实验室进行了12种物质（血红蛋白、白蛋白、转铁蛋白、α（1）-抗胰蛋白酶、纤连蛋白、伊加、IgG、IgM、乳铁蛋白、髓过氧化物酶和中性粒细胞弹性蛋白酶）的生化测定。结果：龈沟液中各物质总量与牙周病状态的关系尚不明确。Logistic回归分析得到6个预测模型，包括物质对：中性粒细胞弹性蛋白酶/伊加、中性粒细胞弹性蛋白酶/血红蛋白、中性粒细胞弹性蛋白酶/α（1）-抗胰蛋白酶和中性粒细胞弹性蛋白酶/IgG、伊加/白蛋白和伊加/转铁蛋白（p < 0.05）。在龈沟液中伊加和中性粒细胞弹性蛋白酶散点图上SPD和SMD的回归线在量的范围内几乎平行。来自这两种物质的预测模型提供的敏感性和特异性分别为88%和94%，respectives.Conclusions：这些结果表明，结合伊加和中性粒细胞弹性蛋白酶在龈沟液中可能是至关重要的牙周疾病状态的预测。此外，这些数据表明，生物化学测定采用这两种物质的龈沟液可以提供一个令人满意的牙周病的筛选试验。
Background: Currently, no biochemical assay involving gingival crevicular fluid is utilized routinely as a screening test for periodontal disease.Objective: The objective of the present study was to evaluate the potential of gingival crevicular fluid assay as a screening methodology.Methods: The subject population was comprised of 27 volunteers. Nine participants were classified as 'subject with periodontal destruction' (SPD) exhibiting at least one site with pocket depth and attachment loss > 3.5 mm, whereas the remaining individuals were categorized as 'subject with minimal periodontal destruction' (SMD). Gingival crevicular fluid was collected from fixed sites via a standardized method. Biochemical assays of 12 substances (hemoglobin, albumin, transferrin, alpha(1)-antitrypsin, fibronectin, IgA, IgG, IgM, lactoferrin, myeloperoxidase and neutrophil elastase) were conducted at a commercial laboratory. Power transformation of total quantities in gingival crevicular fluid was performed for statistical analysis.Results: Relationships between total quantity of each substance and periodontal disease status were unclear. Logistic regression analysis yielded six predictive models, which consisted of substance pairs: neutrophil elastase/IgA, neutrophil elastase/hemoglobin, neutrophil elastase/alpha(1)-antitrypsin and neutrophil elastase/IgG, and IgA/albumin and IgA/transferrin (p < 0.05). Regression lines for SPD and SMD on a scattergram of IgA and neutrophil elastase were nearly parallel within the range of amounts in gingival crevicular fluid. The predictive model derived from both substances afforded sensitivity and specificity of 88% and 94%, respectively.Conclusions: These results indicated that the combination of IgA and neutrophil elastase in gingival crevicular fluid may be crucial for prediction of periodontal disease status. Furthermore, these data suggested that biochemical assays employing both substances in gingival crevicular fluid may provide a satisfactory screening test for periodontal disease.