Improved fluorescence polarization assay for use in evaluating fetal lung maturity. I. Development of the assay procedure.
Improved fluorescence polarization assay for use in evaluating fetal lung maturity. I. Development of the assay procedure.
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改进的荧光偏振测定用于评估胎肺成熟度。
DOI：
10.1093/clinchem/32.2.248
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发表时间：
1986
影响因子：
9.3
通讯作者：
E. Ashwood
中科院分区：
文献类型：
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作者：
J. Tait;R. Franklin;J. B. Simpson;E. Ashwood
我们描述了一种用于预测胎儿肺成熟度的荧光偏振测定法，该方法适用于TDx分析仪（自动荧光偏振仪）。该测定需要0.5mL羊水和约30分钟，并且涉及荧光团1-棕榈酰-2（6-[（7-硝基-2，1，3-苯并恶二唑-4-基）氨基]己酰基）磷脂酰胆碱。可再现的偏振测量依赖于适当的调节孵育时间和温度，但荧光探针和羊水浓度的变化对测量的偏振几乎没有影响，因此对测定精度的影响很小。荧光探针的工作溶液在-20 ° C和pH 5下储存时可稳定至少9个月。干扰物包括红细胞、血清、胆红素、胎粪和利多卡因。
We describe a fluorescence polarization assay for use in predicting fetal lung maturity, which is suitable for the TDx Analyzer (an automated fluorescence polarimeter). The assay requires 0.5 mL of amniotic fluid and approximately 30 min, and involves the fluorophore 1-palmitoyl-2(6-[(7-nitro-2, 1, 3-benzoxadiazol-4-yl)amino]caproyl) phosphatidylcholine. Reproducible polarization measurements depend on proper regulation of incubation time and temperature, but variations in the concentrations of fluorescent probe and amniotic fluid have little effect on measured polarization and therefore little effect on assay precision. Working solutions of the fluorescent probe are stable for at least nine months when stored at -20 degrees C and pH 5. Interferences include erythrocytes, serum, bilirubin, meconium, and lidocaine.
将荧光磷脂酰丝氨酸插入红细胞的质膜中。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Tanaka,Y;Schroit,AJ
通讯作者：
Schroit,AJ