In vivo studies of dialysis-related endotoxemia and bacteremia.
In vivo studies of dialysis-related endotoxemia and bacteremia.
复制标题
透析相关内毒素血症和菌血症的体内研究。
作者：
J. J. Bernick;F. Port;M. Favero
监测犬血液透析过程中细菌和内毒素的转移及发热情况。透析液中的细菌和内毒素水平超过了据报道与热原反应相关的水平，并且在2只狗中静脉注射的无细菌透析液滤液显示出热原。在18次中空纤维透析中，静脉和动脉血液样本中均未检测到鲎试剂（LLA）测定的细菌和内毒素。在透析期间或透析后1/2小时内体温未升高。在平行透析器的实验中，污染的透析液同时被超滤到第二个血室。超滤液在LLA中无反应，在5个实验中，有5个实验用热原试验（USP）证实了不存在热原。本研究提示中空纤维透析器的完整玻璃纸膜是一种有效的内毒素和细菌屏障。
The transfer of bacteria and endotoxin and the development of fever was monitored during hemodialysis of dogs. Bacterial and endotoxin levels in dialysis fluids exceeded those reportedly associated with pyrogenic reactions, and bacteria-free dialysate filtrates administered intravenously were shown to be pyrogenic in 2 of 2 dogs. During 18 hollow-fiber dialyses, neither bacteria nor endotoxin, as measured in the Limulus lysate assay (LLA), was detected in venous and arterial blood samples. Body temperatures did not increase during or within 1/2 h after dialysis. In experiments with parallel dialyzers, contaminated dialysate was simultaneously ultrafiltered into the second blood compartment. Ultrafiltrates were unreactive in the LLA and the absence of pyrogens was confirmed with the pyrogen test, USP, in five of five experiments. This investigation suggests that the intact cellophane membrane of the hollow-fiber dialyzer is an effective barrier to endotoxin and bacteria.