Cell communication with the neural plate is required for induction of neural markers by BMP inhibition: evidence for homeogenetic induction and implications for Xenopus animal cap and chick explant assays.
Cell communication with the neural plate is required for induction of neural markers by BMP inhibition: evidence for homeogenetic induction and implications for Xenopus animal cap and chick explant assays.
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DOI：
10.1016/j.ydbio.2008.12.034
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发表时间：
2009-03-15
影响因子：
2.7
通讯作者：
Stern, Claudio D.
中科院分区：
文献类型：
--
作者：
Linker, Claudia;De Almeida, Irene;Papanayotou, Costis;Stower, Matthew;Sabado, Virginie;Ghorani, Ehsan;Streit, Andrea;Mayor, Roberto;Stern, Claudio D.
关键词：
在非洲爪哇，动物的帽子对BMP拮抗剂非常敏感，这会导致神经化。然而，在鸡中，只有神经板边缘的细胞可以被BMP抑制而神经化。在这里，我们对这两个系统进行比较。BMP拮抗剂可在非洲爪哇腹侧表皮和非神经性鸡上皮细胞中诱导神经板边界标记。然而，只有当BMP抑制的细胞形成一条连接到神经板或其边界的连续轨迹时，BMP拮抗剂才能使外胚层细胞神经化，这表明同源神经化因子只能在BMP抑制的细胞之间移动。非洲爪哇动物的帽外植体含有注定要对神经板边缘甚至前神经板做出贡献的细胞，这解释了为什么它们如此容易被BMP抑制而神经化。此外，从胚胎上胚层分离出的鸡外植体表现出类似非洲爪哇动物帽子的行为，并表达边界标记。我们认为，非洲爪哇的动物帽试验和雏鸡的外植体试验不适合研究神经诱导中的指示信号。
In Xenopus, the animal cap is very sensitive to BMP antagonists, which result in neuralization. In chick, however, only cells at the border of the neural plate can be neuralized by BMP inhibition. Here we compare the two systems. BMP antagonists can induce neural plate border markers in both ventral Xenopus epidermis and non-neural chick epiblast. However, BMP antagonism can only neuralize ectodermal cells when the BMP-inhibited cells form a continuous trail connecting them to the neural plate or its border, suggesting that homeogenetic neuralizing factors can only travel between BMP-inhibited cells. Xenopus animal cap explants contain cells fated to contribute to the neural plate border and even to the anterior neural plate, explaining why they are so easily neuralized by BMP-inhibition. Furthermore, chick explants isolated from embryonic epiblast behave like Xenopus animal caps and express border markers. We propose that the animal cap assay in Xenopus and explant assays in the chick are unsuitable for studying instructive signals in neural induction.
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