Low-density Taqman miRNA array reveals miRNAs differentially expressed in prostatic stem cells and luminal cells.
Low-density Taqman miRNA array reveals miRNAs differentially expressed in prostatic stem cells and luminal cells.
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DOI：
10.1002/pros.21064
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发表时间：
2010-02-15
期刊：
影响因子：
2.8
通讯作者：
Xin, Li
中科院分区：
文献类型：
--
作者：
Zhang, Li;Zhao, Wenping;Valdez, Joseph M.;Creighton, Chad J.;Xin, Li
miRNA 是一类天然存在的小 RNA，通常抑制基因表达。它们已被证明可以积极控制多种生物过程，包括干细胞分化和谱系定型。利用荧光激活细胞分选来分离小鼠前列腺干细胞和分化的管腔细胞。通过定量 RT-PCR 评估了 miRNA 成熟的两种关键 RNAseIII 酶 Drosha 和 Dicer1 的表达水平。还进行了低密度 Taqman miRNA 阵列分析，以鉴定在各个谱系中差异表达的 miRNA。 Drosha 和 Dicer1 在小鼠前列腺干细胞和分化的管腔细胞中以相当的转录水平表达。发现 29 种 miRNA 在前列腺干细胞和管腔细胞中差异表达。许多这些 miRNA 都是以簇的形式编码的，这表明存在细胞特异性的转录调控。据报道，其中一些差异表达的 miRNA 可以调节与每个谱系的分子和表型特征相关的基因。 miRNA 可能在前列腺谱系特性的精细调节中发挥潜在的关键作用。
miRNAs are a class of naturally occurring small RNAs that generally repress gene expression. They have been shown to actively control diverse biological processes including stem cell differentiation and lineage commitment. Fluorescence-activated cell sorting was utilized to isolate murine prostate stem cells and differentiated luminal cells. The expression levels of Drosha and Dicer1, the two key RNAseIII enzymes for miRNA maturation, were evaluated by quantitative RT-PCR. Low-density Taqman miRNA array analyses were also performed to identify miRNAs that are differentially expressed in individual lineages. Drosha and Dicer1 are expressed at comparable transcriptional levels in murine prostate stem cells and differentiated luminal cells. Twenty-nine miRNAs were discovered to be differentially expressed in prostate stem cells and luminal cells. Many of these miRNAs are coded in clusters, suggesting a cell-specific transcriptional regulation. Some of these differentially expressed miRNAs have been reported to regulate genes relevant to the molecular and phenotypic features of each lineage. miRNAs may play a potentially critical role in fine regulation of prostatic lineage identity.
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