Functional profiling of adenylation domains in nonribosomal peptide synthetases by competitive activity-based protein profiling
Functional profiling of adenylation domains in nonribosomal peptide synthetases by competitive activity-based protein profiling
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DOI：
10.1039/c5cc04953a
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发表时间：
2015-01-01
影响因子：
4.9
通讯作者：
Kakeya, Hideaki
中科院分区：
文献类型：
--
作者：
Kasai, Shota;Konno, Sho;Kakeya, Hideaki
我们描述了基于竞争活性的蛋白质谱分析（ABPP），以加速非核糖体肽合成酶（NRPS）在蛋白质组环境中的腺苷酸化（A）结构域的功能预测和评估。使用氨磺酰氧基连接的氨酰-AMP类似物的库，竞争性ABPP技术提供了一个简单而快速的腺苷酸化酶的测定系统，并提供了深入了解酶底物候选人和酶活性位点结构。
We describe competitive activity-based protein profiling (ABPP) to accelerate the functional prediction and assessment of adenylation (A) domains in nonribosomal peptide synthetases (NRPSs) in proteomic environments. Using a library of sulfamoyloxy-linked aminoacyl-AMP analogs, the competitive ABPP technique offers a simple and rapid assay system for adenylating enzymes and provides insight into enzyme substrate candidates and enzyme active-site architecture.