A disposable microfluidic biochip with on-chip molecularly imprinted biosensors for optical detection of anesthetic propofol
A disposable microfluidic biochip with on-chip molecularly imprinted biosensors for optical detection of anesthetic propofol
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DOI：
10.1016/j.bios.2010.01.037
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发表时间：
2010-05-15
影响因子：
12.6
通讯作者：
Hong, Chian-Lang
中科院分区：
文献类型：
--
作者：
Hong, Chien-Chong;Chang, Po-Hsiang;Hong, Chian-Lang
本文介绍了一种一次性微流控生物芯片，其上带有分子印迹生物传感器，用于麻醉药异丙酚的光学检测。目前，医院检测麻醉药异丙酚的方法主要有液相色谱法（LC）、高效液相色谱法（HPLC）和气相色谱-质谱法（GC-MS）。这些传统的仪器体积庞大、价格昂贵，并且不容易接近。在这项工作中，一种新的塑料微流控生物芯片与芯片上的麻醉生物传感器已被开发和特点，用于快速检测麻醉异丙酚。将模板分子印迹聚合物组装到微流控生物芯片中，利用丙泊酚与显色剂反应，在655 nm波长处检测麻醉药物丙泊酚。实验结果表明，微流控生物芯片的灵敏度为6.47 mV/（ppm mm（2））。MIP与非印迹聚合物（NIP）的特异结合率高达456%。该微系统的检出限为0.25ppm，线性范围为0.25 ~ 10 ppm。本文提出的基于分子印迹聚合物的一次性微流控生物芯片对麻醉药物异丙酚分子的分离和检测具有良好的性能。与传统的大规模仪器相比，微流控生物芯片具有体积小、灵敏度高、选择性好、成本低、响应速度快等优点。(C)2010 Elsevier B. V.保留所有权利。
This paper presents a disposable microfluidic biochip with on-chip molecularly imprinted biosensors for optical detection of anesthetic propofol. So far, the methods to detect anesthetic propofol in hospitals are liquid chromatography (LC), high-performance liquid chromatography (HPLC), and gas chromatography-mass spectroscopy (GC-MS). These conventional instruments are bulky, expensive, and not ease of access. In this work, a novel plastic microfluidic biochip with on-chip anesthetic biosensor has been developed and characterized for rapid detection of anesthetic propofol. The template-molecule imprinted polymers were integrated into microfluidic biochips to be used for detecting anesthetic propofol optically at 655 nm wavelength after the reaction of propofol with color reagent. Experimental results show that the sensitivity of the microfluidic biochip with on-chip molecularly imprinted polymers (MIPs) biosensor is 6.47 mV/(ppm mm(2)). The specific binding of MIP to non-imprinted polymer (NIP) is up to 456%. And the detection limit of the microsystem is 0.25 ppm with a linear detection range from 0.25 to 10 ppm. The disposable microfluidic biochip with on-chip anesthetic biosensor using molecularly imprinted polymers presented in this work showed excellent performance in separation and sensing of anesthetic propofol molecules. While compared to large-scale conventional instruments, the developed microfluidic biochips with on-chip MIP biosensors have the advantages of compact size, high sensitivity, high selectivity, low cost, and fast response. (C) 2010 Elsevier B.V. All rights reserved.