Detection of high molecular weight vinculin binding proteins in muscle and nonmuscle tissues with an electroblot-overlay technique.
Detection of high molecular weight vinculin binding proteins in muscle and nonmuscle tissues with an electroblot-overlay technique.
复制标题
使用电印迹叠加技术检测肌肉和非肌肉组织中的高分子量纽蛋白结合蛋白。
DOI：
10.1016/s0006-291x(83)80245-9
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发表时间：
1983
影响因子：
3.1
通讯作者：
Lin,S
中科院分区：
文献类型：
--
作者：
Wilkins,JA;Chen,KY;Lin,S
在这项研究中，我们研究了结合放射性标记的纽蛋白的蛋白质分离的十二烷基硫酸钠-聚丙烯酰胺凝胶，然后转移到硝酸纤维素片。我们检测到黏着斑蛋白的饱和结合的多肽与明显的先生的215，000，205，000和185，000在一个低离子强度提取物从鸡砂囊膜。还检测到黏着斑蛋白与人血小板中表观Mr为205，000、185，000和165，000的蛋白质的结合。此外，我们还发现[125 I]黏着斑蛋白与未标记黏着斑蛋白和α-辅肌动蛋白结合，尽管这些相互作用的亲和力似乎低于与高分子量蛋白的相互作用。
In this study, we examined binding of radiolabelled vinculin to proteins separated on sodium dodecyl sulfate-polyacrylamide gels, and then electrophoretically transferred onto nitrocellulose sheets. We detected saturable binding of vinculin to polypeptides with apparent Mr's of 215,000, 205,000 and 185,000 in a low ionic strength extract from chicken gizzard membranes. Binding of vinculin to proteins with apparent Mr's of 205,000, 185,000, and 165,000 in human platelets was also detected. In addition, we found that [125I]vinculin binds to unlabelled vinculin and to α-actinin, although these interactions appear to be of lower affinity than those with the higher molecular weight proteins.