Violaxanthin de-epoxidase, the xanthophyll cycle enzyme, requires lipid inverted hexagonal structures for its activity
Violaxanthin de-epoxidase, the xanthophyll cycle enzyme, requires lipid inverted hexagonal structures for its activity
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DOI：
10.1021/bi049652g
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发表时间：
2004-04-20
期刊：
影响因子：
2.9
通讯作者：
Strzalka, K
中科院分区：
文献类型：
--
作者：
Latowski, D;Åkerlund, HE;Strzalka, K
双层形成脂质对维持紫黄质去环氧酶的酶活性无效。另一方面，不形成双层的脂质，无论其化学性质如何，都保证了紫黄质去环氧化酶的高活性，从而使紫黄质转化为玉米黄质。我们的数据表明，脂质形成反六边形结构的存在是必需的紫黄质去环氧酶的活性，这种活性取决于脂肪酸的不饱和程度。讨论了反六方相域在模型体系和天然类囊体膜中紫黄质转化为玉米黄质过程中的意义。
Bilayer-forming lipids were shown to be ineffective in sustaining the enzymatic activity of violaxanthin de-epoxidase. On the other hand, non-bilayer-forming lipids, regard-less of their different chemical character, ensured high activity of violaxanthin de-epoxidase, resulting in conversion of violaxanthin to zeaxanthin. Our data indicates that the presence of lipids forming reversed hexagonal structures is necessary for violaxanthin de-epoxidase activity and this activity is dependent on the degree of unsaturation of the fatty acids. The significance of the reversed hexagonal phase domains in the conversion of violaxanthin into zeaxanthin in model systems and in the native thylakoid membranes is discussed.