Neutralization of HTLV-III/LAV replication by antiserum to thymosin alpha 1.
Neutralization of HTLV-III/LAV replication by antiserum to thymosin alpha 1.
复制标题
胸腺素 α 1 抗血清中和 HTLV-III/LAV 复制。
DOI：
10.1126/science.3010464
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Goldstein,AL
中科院分区：
文献类型：
--
作者：
Sarin,PS;Sun,DK;Thornton,AH;Naylor,PH;Goldstein,AL
抗胸腺素α1（胸腺分泌的一种激素）制备的抗血清可有效中和AIDS相关病毒[HTLV-III/LAV（克隆BH-10）]，并阻断其在H9细胞中的复制。逆转录酶活性和HTLV-III/LAV抗原p15和p24的表达被纯化的胸腺素α1抗血清免疫球蛋白G制剂抑制。抗血清的抗病毒活性是由于胸腺素α 1与HTLV-III/LAV gag基因产物p17之间的同源性。比较胸腺素α 1和gag蛋白的一级序列，发现胸腺素α 1的第11和28位与gag蛋白的第92和109位之间的18个氨基酸的同源性为44%至50%。胸腺素α 1抗血清和富含免疫球蛋白G的制剂在阻断H9细胞中HTLV-III（BH-10）复制方面的有效性表明了开发艾滋病疫苗的新方法。针对gag蛋白的疫苗可能克服病毒包膜区域的遗传漂移问题，并可用于对抗HTLV-III/LAV的所有遗传变异。
An antiserum prepared against thymosin α1, a hormone secreted by the thymus gland, effectively neutralized the AIDS-associated virus [HTLV-III/LAV (clone BH-10)] and blocked its replication in H9 cells. Reverse transcriptase activity and expression of the HTLV-III/LAV antigens p15 and p24 were inhibited by purified immunoglobulin G preparations of antisera to thymosin α1. The antiviral activity of the antiserum was found to be due to a region of homology between thymosin α1and p17, a product of thegaggene of HTLV-III/LAV. Comparison of the primary sequences of thymosin α1and thegagprotein revealed a 44% to 50% homology in an 18-amino acid region, between positions 11 and 28 on thymosin α1and 92 and 109 on thegagprotein. The effectiveness of the thymosin α1antiserum and of immunoglobulin G-enriched preparations in blocking replication of HTLV-III(BH-10) in H9 cells suggests a novel approach to the development of an AIDS vaccine. A vaccine directed against thegagprotein might overcome the problem of genetic drift in the envelope region of the virus and be useful against all genetic variants of HTLV-III/LAV.