A rapid genotyping method for the vivax malaria transmission-blocking vaccine candidates, Pvs25 and Pvs28
A rapid genotyping method for the vivax malaria transmission-blocking vaccine candidates, Pvs25 and Pvs28
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DOI：
10.1016/j.parint.2004.01.012
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发表时间：
2004-09-01
影响因子：
1.9
通讯作者：
Torii, M
中科院分区：
文献类型：
--
作者：
Tsuboi, T;Kaneko, O;Torii, M
在许多候选疫苗中观察到的抗原多样性是设计有效的疟疾疫苗的困难之一。由于调查疫苗候选抗原的基因多态性是疫苗研发的前提，因此有必要建立高效的筛选方法来从大量样本中检测基因多态性。在这里，我们建立了高效的聚合酶链反应-单链构象多态性（PCR-SSCP）方法来检测疟疾传播候选疫苗Pvs25和Pvs28的核苷酸多样性。我们可以通过这种方法区分 Pvs25 的所有 4 个单倍型。通过引入 Pvs28 的 BsmI 消化步骤，我们可以通过单次电泳区分 15/16 单倍型。由于该方法既不需要测序也不需要放射性同位素标记，因此很容易将该方法转移到基于现场的遗传多态性高通量筛选中。 (C) 2004 Elsevier Ireland Ltd. 保留所有权利。
The antigenic diversity observed in many vaccine candidates is one of the difficulties to design effective malaria vaccine. Since it is prerequisite to survey genetic polymorphism of the vaccine candidate antigens for the vaccine development, it is necessary to establish efficient screening method to detect the genetic polymorphism from a large number of samples. Here, we have established efficient polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) method to detect nucleotide diversity of the malaria transmission-blocking vaccine candidates Pvs25 and Pvs28. We can distinguish all 4 haplotypes of Pvs25 by this method. By introducing BsmI-digestion step for Pvs28, we can distinguish 15/16 haplotypes by single electrophoresis. Since this method requires neither sequencing nor radioisotope labeling, it will be easy to transfer the method into a field based high throughput screening of genetic polymorphism. (C) 2004 Elsevier Ireland Ltd. All rights reserved.