Cerebellum encodes and influences the initiation, performance, and termination of discontinuous movements in mice.
Cerebellum encodes and influences the initiation, performance, and termination of discontinuous movements in mice.
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小脑编码并影响小鼠不连续运动的启动、表现和终止。
DOI：
10.7554/elife.71464
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发表时间：
2022-04-22
期刊：
影响因子：
7.7
通讯作者：
Ito-Ishida, Aya
中科院分区：
文献类型：
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作者：
Gaffield, Michael A.;Sauerbrei, Britton A.;Christie, Jason M.;Ito-Ishida, Aya
假设小脑代表在周期性执行的不连续运动期间组织显著运动事件的重要计时信息。为了提供证实这一想法的功能证据，我们测量并操纵了小鼠小脑外侧浦肯野细胞(PC)的活动，这些小鼠被训练成自愿地进行周期性的舔食，以获得定期分配的水奖励。总体而言，PC简单的尖峰电位在任务执行过程中受到调制，绘制了舌头伸出和收回的相位图，以及舔咬开始和结束之前的斜坡，这两个重要的运动事件划定了运动周期。倾斜的开始发生在无提示的探索性舔的开始，即预期相对于舔的水的可获得性，这是对水分配的反应，这表明小脑参与的方式取决于运动环境。在PC的一个亚群中，爬升纤维诱发的反应在舔咬开始时也会增加，但不会终止，这突显了小脑输入路径如何代表与任务相关的信息的差异。PC活动的光遗传扰动通过降低舔咬节律性来扰乱行为，此外，还通过开始和终止舔舔回合确认了在运动组织中的因果作用。综上所述，这些结果证实了小脑对重复的运动动作的启动和计时有贡献。
The cerebellum is hypothesized to represent timing information important for organizing salient motor events during periodically performed discontinuous movements. To provide functional evidence validating this idea, we measured and manipulated Purkinje cell (PC) activity in the lateral cerebellum of mice trained to volitionally perform periodic bouts of licking for regularly allocated water rewards. Overall, PC simple spiking modulated during task performance, mapping phasic tongue protrusions and retractions, as well as ramping prior to both lick-bout initiation and termination, two important motor events delimiting movement cycles. The ramping onset occurred earlier for the initiation of uncued exploratory licking that anticipated water availability relative to licking that was reactive to water allocation, suggesting that the cerebellum is engaged differently depending on the movement context. In a subpopulation of PCs, climbing-fiber-evoked responses also increased during lick-bout initiation, but not termination, highlighting differences in how cerebellar input pathways represent task-related information. Optogenetic perturbation of PC activity disrupted the behavior by degrading lick-bout rhythmicity in addition to initiating and terminating licking bouts confirming a causative role in movement organization. Together, these results substantiate that the cerebellum contributes to the initiation and timing of repeated motor actions.