Identification of lactococci and enterococci by colony hybridization with 23S rRNA-targeted oligonucleotide probes
Identification of lactococci and enterococci by colony hybridization with 23S rRNA-targeted oligonucleotide probes
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通过与 23S rRNA 靶向寡核苷酸探针进行菌落杂交来鉴定乳球菌和肠球菌
DOI：
10.1128/aem.56.9.2927-2929.1990
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发表时间：
1990
影响因子：
4.4
通讯作者：
K. Schleifer
中科院分区：
文献类型：
--
作者：
Wolfgang Ludwig;K. Schleifer
鉴定了乳酸乳球菌、粪肠球菌、屎肠球菌和恶臭肠球菌/鸟肠球菌的23S rRNA的特定序列，并合成了互补寡核苷酸探针。通过斑点印迹和菌落杂交评估探针的特异性。该探针可用于混合培养物中相应物种菌落的特异性检测和鉴定。
Specific sequences of 23S rRNA of Lactococcus lactis, Enterococcus faecalis, Enteroccus faecium, and Enterococcus malodoratus/Enterococcus avium were identified, and complementary oligonucleotide probes were synthesized. The specificity of the probes was evaluated by dot blot and colony hybridizations. The probes can be used for the specific detection and identification of colonies of the corresponding species in mixed cultures.