Wide and Deep Imaging of Neuronal Activities by a Wearable NeuroImager Reveals Premotor Activity in the Whole Motor Cortex
Wide and Deep Imaging of Neuronal Activities by a Wearable NeuroImager Reveals Premotor Activity in the Whole Motor Cortex
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DOI：
10.1038/s41598-019-44146-x
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发表时间：
2018-10
影响因子：
4.6
通讯作者：
Takuma Kobayashi;T. Islam;Masaaki Sato;Masamichi Ohkura;J. Nakai;Y. Hayashi;H. Okamoto
中科院分区：
文献类型：
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作者：
Takuma Kobayashi;T. Islam;Masaaki Sato;Masamichi Ohkura;J. Nakai;Y. Hayashi;H. Okamoto
在自由活动的动物身上进行功能性全脑成像的可穿戴技术将促进我们对认知过程和适应行为的理解。荧光成像可以在真实的时间内可视化单个神经元的活动，但传统的显微镜在宽度和景深上都具有有限的样本覆盖范围。在这里，我们开发了一种新型的头戴式激光相机（HLC），具有微距和深焦镜头，能够以细胞分辨率进行荧光成像，用于表达层和细胞类型特异性钙探针的小鼠的全面成像。我们可视化的方向选择性在个别兴奋性神经元在整个视觉皮层的一个半球，和细胞组装表达运动前活动之前，自愿运动在整个运动皮层的两个半球。包括多路复用和无线接口的选项，我们的可穿戴，广泛和深入的成像HLC技术可以实现简单和经济的神经元群体的认知和行为的映射。
Wearable technologies for functional whole brain imaging in freely moving animals would advance our understanding of cognitive processing and adaptive behavior. Fluorescence imaging can visualize the activity of individual neurons in real time, but conventional microscopes have limited sample coverage in both the width and depth of view. Here we developed a novel head-mounted laser camera (HLC) with macro and deep-focus lenses that enable fluorescence imaging at cellular resolution for comprehensive imaging in mice expressing a layer- and cell type-specific calcium probe. We visualized orientation selectivity in individual excitatory neurons across the whole visual cortex of one hemisphere, and cell assembly expressing the premotor activity that precedes voluntary movement across the motor cortex of both hemispheres. Including options for multiplex and wireless interfaces, our wearable, wide- and deep-imaging HLC technology could enable simple and economical mapping of neuronal populations underlying cognition and behavior.