MICROSATELLITE INSTABILITY IN CANCER OF THE PROXIMAL COLON
MICROSATELLITE INSTABILITY IN CANCER OF THE PROXIMAL COLON
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DOI：
10.1126/science.8484122
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发表时间：
1993-05-07
期刊：
影响因子：
56.9
通讯作者：
SCHAID, D
中科院分区：
文献类型：
--
作者：
THIBODEAU, SN;BREN, G;SCHAID, D
检查结直肠肿瘤 DNA 人类染色体 5q、15q、17p 和 18q 上 (CA)n 重复序列的体细胞不稳定性。在检查的 90 个肿瘤中，有 25 个（28%）检测到了肿瘤 DNA 与正常 DNA 之间的差异。这种不稳定性表现为重复长度的重大变化（通常本质上是异质的）或微小的变化（通常是两个碱基对）。微卫星不稳定性与肿瘤在近端结肠的位置显着相关（P = 0.003），与患者存活率的增加（P = 0.02）显着相关，并且与染色体 5q、17p 和 18q 杂合性的丧失呈反比。这些数据表明，某些结直肠癌可能是通过不一定涉及杂合性丧失的机制产生的。
Colorectal tumor DNA was examined for somatic instability at (CA)n repeats on human chromosomes 5q, 15q, 17p, and 18q. Differences between tumor and normal DNA were detected in 25 of the 90 (28 percent) tumors examined. This instability appeared as either a substantial change in repeat length (often heterogeneous in nature) or a minor change (typically two base pairs). Microsatellite instability was significantly correlated with the tumor's location in the proximal colon (P = 0.003), with increased patient survival (P = 0.02), and, inversely, with loss of heterozygosity for chromosomes 5q, 17p, and 18q. These data suggest that some colorectal cancers may arise through a mechanism that does not necessarily involve loss of heterozygosity.