Genomic expression programs in the response of yeast cells to environmental changes
Genomic expression programs in the response of yeast cells to environmental changes
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DOI：
10.1091/mbc.11.12.4241
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发表时间：
2000-12-01
影响因子：
3.3
通讯作者：
Brown, PO
中科院分区：
文献类型：
--
作者：
Gasch, AP;Spellman, PT;Brown, PO
我们探讨了基因组表达模式的酵母酿酒酵母响应不同的环境转变。DNA微阵列用于测量几乎每个酵母基因的转录水平随时间的变化，因为细胞对温度冲击、过氧化氢、产生超氧化物的药物甲萘醌、巯基氧化剂二酰胺、二硫化物还原剂二硫苏糖醇、高渗和低渗冲击、氨基酸饥饿、氮源耗尽和进入稳定期的进展作出反应。大量基因（约900个）对几乎所有这些环境变化都表现出类似的强烈反应。基因组反应的其他特征专门针对特定条件。启动子分析和随后表征的突变株的反应牵连的转录因子Yap1p，以及Msn2p和Msn4p，在介导的转录反应的特定功能，而新的序列元件的识别提供了线索，新的监管机构。基因组对特定环境压力的反应中的生理学主题提供了对这些压力对细胞影响的见解。
We explored genomic expression patterns in the yeast Saccharomyces cerevisiae responding to diverse environmental transitions. DNA microarrays were used to measure changes in transcript levels over time for almost every yeast gene, as cells responded to temperature shocks, hydrogen peroxide, the superoxide-generating drug menadione, the sulfhydryl-oxidizing agent diamide, the disulfide-reducing agent dithiothreitol, hyper- and hypo-osmotic shock, amino acid starvation, nitrogen source depletion, and progression into stationary phase. A large set of genes (similar to 900) showed a similar drastic response to almost all of these environmental changes. Additional features of the genomic responses were specialized for specific conditions. Promoter analysis and subsequent characterization of the responses of mutant strains implicated the transcription factors Yap1p, as well as Msn2p and Msn4p, in mediating specific features of the transcriptional response, while the identification of novel sequence elements provided clues to novel regulators. Physiological themes in the genomic responses to specific environmental stresses provided insights into the effects of those stresses on the cell.