Biocircuit design through engineering bacterial logic gates
Biocircuit design through engineering bacterial logic gates
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DOI：
10.1007/s11047-010-9184-2
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发表时间：
2011-03-01
影响因子：
2.1
通讯作者：
Castellanos, Juan
中科院分区：
文献类型：
--
作者：
Goni-Moreno, Angel;Redondo-Nieto, Miguel;Castellanos, Juan
设计合成生物电路以实现预期的目的是一个在过去十年中呈指数级增长的科学领域。基因组测序、细菌基因调控网络的进展，以及通过群体感应信号进一步了解种内细菌通信是这项工作的起点。虽然生物电路大多是在单个细胞中发展的，但在这里我们提出了一个模型，其中每个细菌都被认为是一个单一的逻辑门，并且化学细胞到细胞的连接被设计来控制电路的功能。每个逻辑过程都有一个转基因细菌菌株，这将允许我们通过混合种群来开发具有不同行为的电路，而不是对单个菌株内的整个遗传网络进行重新编程。强调了这一过程的两个主要优点。首先，获得了完全连接的电路，其中每个细胞门都能够与所有其他细胞进行通信。第二，对不适当基因表达产生的噪音的抵抗力。最后一个目标是通过对输入信号的阈值建模来实现的。因此，如果输入浓度没有超过阈值，则门的逻辑功能会忽略它。
Designing synthetic biocircuits to perform desired purposes is a scientific field that has exponentially grown over the past decade. The advances in genome sequencing, bacteria gene regulatory networks, as well as the further knowledge of intraspecies bacterial communication through quorum sensing signals are the starting point for this work. Although biocircuits are mostly developed in a single cell, here we propose a model in which every bacterium is considered to be a single logic gate and chemical cell-to-cell connections are engineered to control circuit function. Having one genetically modified bacterial strain per logic process would allow us to develop circuits with different behaviors by mixing the populations instead of re-programming the whole genetic network within a single strain. Two principal advantages of this procedure are highlighted. First, the fully connected circuits obtained where every cellgate is able to communicate with all the rest. Second, the resistance to the noise produced by inappropriate gene expression. This last goal is achieved by modeling thresholds for input signals. Thus, if the concentration of input does not exceed the threshold, it is ignored by the logic function of the gate.