Functional promoter elements common to ribosomal protein and ribosomal RNA genes in Neurospora crassa
Functional promoter elements common to ribosomal protein and ribosomal RNA genes in Neurospora crassa
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DOI：
10.1007/s004380050314
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发表时间：
1996-11-27
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影响因子：
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通讯作者：
Tyler, BM
中科院分区：
文献类型：
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作者：
Cujec, TP;Tyler, BM
利用启动子缺失和替换突变体鉴定了粗糙脉孢菌胞质核糖体蛋白基因（crp-2）的启动子序列。一个基因靶向策略被用来在体内检测突变体。crp-2的启动子结构是复杂的，在小鼠中比在酿酒酵母中更类似于核糖体蛋白基因。六个区域被确定为重要的转录。这些元件包括两个元件，CG重复序列和Dde盒，它们在大多数N. crassa核糖体蛋白基因，并且也已被证明是体外RNA聚合酶I从40 S rRNA启动子转录所需的。位于-73至-66和-189至-154之间的CG重复序列在功能上是冗余的，并且使转录效率提高了10至15倍。位于-153至-147和-95至-83的Dde框分别对转录效率贡献2倍和5倍。在-254和-190之间的未鉴定元件贡献2倍，而在-85和-66之间的富含嘧啶的区域影响转录的起始点。
The promoter sequences of a cytoplasmic ribosomal protein gene (crp-2) of Neurospora crassa were identified using promoter deletion and substitution mutants. A gene-targeting strategy was used to assay the mutants in vivo. The promoter architecture of crp-2 is complex and is more similar to that of ribosomal protein genes in mouse than in Saccharomyces cerevisiae. Six regions were identified as important for transcription. These included two elements, a CG repeat and a Dde box, that are conserved in most other promoters of N. crassa ribosomal protein genes and have also been demonstrated as being required for transcription from the 40 S rRNA promoter by RNA polymerase I in vitro. The CG repeats located at -73 to -66 and between -189 and -154 were functionally redundant and increased transcription efficiency by 10- to 15-fold. The Dde boxes located at -153 to -147 and at -95 to -83 contributed 2-fold and 5-fold to transcription efficiency, respectively. An unidentified element between -254 and -190 contributed 2-fold, while a pyrimidine-rich region between -85 and -66 influenced the start point of transcription.