Interplay between nutrient transporters ensures fertility in the malaria mosquito Anopheles gambiae.
Interplay between nutrient transporters ensures fertility in the malaria mosquito Anopheles gambiae.
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营养转运蛋白之间的相互作用确保了疟疾冈比亚按蚊的生育能力。
DOI：
10.1101/2023.06.02.543516
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Catteruccia,Flaminia
中科院分区：
文献类型：
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作者：
Stryapunina,Iryna;Itoe,Maurice;Trinh,Queenie;Vidoudez,Charles;Du,Esrah;Mendoza,Lydia;Hulai,Oleksandr;Kauffman,Jamie;Carew,John;Shaw,WilliamRobert;Catteruccia,Flaminia
许多蚊子的雌性以血液为食，以获得卵发育所需的营养。卵发生周期的特征在于虫媒病毒载体埃及伊蚊，其中在血餐后，脂质转运蛋白（Lp）将脂质从中肠和脂肪体运送到卵巢，并且卵黄前体蛋白，卵黄蛋白原（Vg）通过受体介导的内吞作用沉积到卵母细胞中。然而，我们对这两种营养转运蛋白的作用如何相互协调的理解在这种蚊子和其他蚊子物种中是有限的。在这里，我们证明，在疟疾蚊子冈比亚按蚊，Lp和Vg的调节是及时的，以优化卵子发育，并确保生育力。通过Lp沉默的脂质转运缺陷触发卵巢卵泡发育失败，导致Vg和异常的卵黄颗粒的失调。相反，Vg的消耗导致脂肪体中Lp的上调，其方式似乎至少部分依赖于雷帕霉素靶（TOR）信号传导，导致发育中的卵泡中过量脂质积累。由缺乏Vg的母亲沉积的胚胎是完全不育的，并且在发育早期被阻止，这可能是由于氨基酸水平和蛋白质合成严重降低。我们的研究结果表明，这两种营养转运蛋白的相互调节是必不可少的，以保障生育能力，确保正确的营养平衡，在发展中的卵母细胞，并验证Vg和Lp作为两个潜在的候选人，蚊子控制。
Females from many mosquito species feed on blood to acquire nutrients for egg development. The oogenetic cycle has been characterized in the arboviral vector Aedes aegypti, where after a bloodmeal, the lipid transporter lipophorin (Lp) shuttles lipids from the midgut and fat body to the ovaries, and a yolk precursor protein, vitellogenin (Vg), is deposited into the oocyte by receptor-mediated endocytosis. Our understanding of how the roles of these two nutrient transporters are mutually coordinated is however limited in this and other mosquito species. Here, we demonstrate that in the malaria mosquito Anopheles gambiae, Lp and Vg are reciprocally regulated in a timely manner to optimize egg development and ensure fertility. Defective lipid transport via Lp silencing triggers abortive ovarian follicle development, leading to misregulation of Vg and aberrant yolk granules. Conversely, depletion of Vg causes an upregulation of Lp in the fat body in a manner that appears to be at least partially dependent on target of rapamycin (TOR) signaling, resulting in excess lipid accumulation in the developing follicles. Embryos deposited by Vg-depleted mothers are completely infertile, and are arrested early during development, likely due to severely reduced amino acid levels and protein synthesis. Our findings demonstrate that the mutual regulation of these two nutrient transporters is essential to safeguard fertility by ensuring correct nutrient balance in the developing oocyte, and validate Vg and Lp as two potential candidates for mosquito control.