Prominent expression of transforming growth factor β2 gene in the chicken embryonic gonad as revealed by suppressive subtraction cloning
Prominent expression of transforming growth factor β2 gene in the chicken embryonic gonad as revealed by suppressive subtraction cloning
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DOI：
10.1006/gcen.2001.7719
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发表时间：
2002-02-01
影响因子：
2.7
通讯作者：
Fujihara, N
中科院分区：
文献类型：
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作者：
Hattori, M;Furuta, H;Fujihara, N
从鸡胚胎性腺的脑，心脏，肝脏，砂囊，中肾，和肌肉的组织中减去cDNA克隆进行鉴定与胚胎卵巢和睾丸表达独特的生长因子基因。我们获得了几个编码已知和许多未知基因的cDNA克隆。我们首次发现转化生长因子β 2（TGF-β 2）在鸡胚卵巢和睾丸中优先表达。通过对其他胚胎组织的逆转录-聚合酶链反应分析，进一步分析了卵巢和睾丸中TGF-β 2选择性表达的cDNA消减克隆。在鸡胚胎卵巢和睾丸中评估TGF-β 2的个体发生。在睾丸和卵巢中，TGF-β 2的转录水平在胚胎发育早期（U）较高，到胚胎后期（E14-E17）逐渐下降，然后在胚胎最后一天（E21）略有上升。在左右卵巢之间，每个RNA的TGF-β 2转录物没有差异。TGF-β 2可能在胚胎期鸡卵巢和睾丸生殖细胞发育的调节中起关键作用。(C)2002 Elsevier Science（美国）。
cDNA cloning from chicken embryonic gonad subtracted from tissues of the brain, heart, liver, gizzard, mesonephros, and muscle was performed to identify growth factor genes with expression unique to embryonic ovary and testis. We obtained several cDNA clones encoding known and many unknown genes. We found for the first time that the transforming growth factor beta2 (TGF-beta2) is preferentially expressed in the chicken embryonic ovary and testis. cDNA subtraction cloning with respect to the selective expression of TGF-beta2 in the ovary and testis was further analyzed by reverse transcription-polymerase chain reaction analyses of other embryonic tissues. The ontogeny of TGF-beta2 was evaluated in chicken embryonic ovary and testis. in both testis and ovary, the levels of TGF-beta2 transcripts were high during the early period of embryonic development (U), gradually decreased until the late embryonic days (E14-E17), and then slightly increased at the last embryonic day (E21). There was no difference in the TGF-beta2 transcripts per RNA between the left and the right ovaries. TGF-beta2 may have a critical role in the regulation of the development of chicken ovarian and testicular germ cells during the embryonic period. (C) 2002 Elsevier Science (USA).