DNA Hybridization Assay Using Gold Nanoparticles and Electrophoresis Separation Provides 1 pM Sensitivity
DNA Hybridization Assay Using Gold Nanoparticles and Electrophoresis Separation Provides 1 pM Sensitivity
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DOI：
10.1021/acs.bioconjchem.7b00682
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发表时间：
2018-01-01
影响因子：
4.7
通讯作者：
Saiki, Toshiharu
中科院分区：
文献类型：
--
作者：
Esashika, Keiko;Saiki, Toshiharu
通过检查几种可能的杂交组合，最大限度地提高了由两个探针 DNA 分子和互补靶 DNA 分子之间的杂交介导的金纳米颗粒 (AuNP) 二聚化的效率。 AuNP 大小的均匀性、适合高杂交效率的表面修饰的使用以及非特异性结合的有效阻断，都有助于在 DNA 修饰的 AuNP 多聚体的常规凝胶电泳分离后实现 1 pM 的检测限。这种实用的同质 DNA 杂交测定方法将为临床诊断提供快速、经济高效且现场便携式的工具。
The efficiency of gold nanoparticle (AuNP) dimerization mediated by hybridization between two probe DNA molecules and a complementary target DNA molecule was maximized by examining several possible hybridization combinations. The uniformity of the size of the AuNPs, the use of surface modification appropriate for high hybridization efficiency, together with efficient blocking of nonspecific binding, all contributed to achieving a 1 pM detection limit following conventional gel electrophoresis separation of the DNA-modified AuNP multimers. This practical homogeneous DNA hybridization assay methodology will provide a rapid, cost-effective, and field-portable tool for clinical diagnosis.