Slice blotting: a method for detecting the release of immunoreactive substances from living brain tissue.
Slice blotting: a method for detecting the release of immunoreactive substances from living brain tissue.
复制标题
切片印迹：一种检测活体脑组织释放免疫反应物质的方法。
DOI：
10.1016/s0165-0270(99)00074-6
复制
发表时间：
1999
影响因子：
3
通讯作者：
Lowe,G
中科院分区：
文献类型：
--
作者：
Lowe,G
中枢神经系统的复杂电路形成了组织严密的结构，其中包含大量以特定模式和途径表达的发射器、调制器和其他化学信号。用免疫组织化学和分子生物学技术进行的解剖学研究已经详细地描绘了这些物质在固定组织中的分布。然而，神经活性物质的释放往往受到精确的空间控制，并受到众多生理因素的调节。了解这种复杂的细胞间信号系统将需要开发新的空间分辨方法来检测生命系统中的分泌。这里描述了一种简单但强大的方法，用于可视化和量化从活的神经组织释放的化学信号的时间整合的空间模式。该方法将体外脑片制备与免疫印迹检测抗原的方法相结合。它在生物学研究中具有广泛的潜在应用，因为它可以绘制细胞因子、生长因子、趋化物质、化学物质、形态因子、酶和其他旁分泌信号在空间组织系统中的体外释放模式，受到各种刺激和条件的影响。
The intricate circuitry of the CNS forms highly organized structures containing a multitude of transmitters, modulators and other chemical signals expressed in specific patterns and pathways. Anatomical studies with immunohistochemical and molecular biological techniques have mapped in fine detail the distribution of these substances in fixed tissue. However, the release of neuroactive substances is often under precise spatial control and is regulated by numerous physiological factors. Understanding such complex intercellular signaling systems will require the development of new spatially resolved methods for detecting secretion in living systems. A simple but powerful method is described here for visualizing and quantifying the time-integrated spatial pattern of release of chemical signals from living neural tissue. The method combines the in vitro brain slice preparation with immunostaining protocols used for antigen detection on Western blots. It has widespread potential application in biological research because it can map in vitro patterns of release of cytokines, growth factors, chemoattractants, chemorepellents, morphogens, enzymes, and other paracrine signals in spatially organized systems, subject to a variety of stimuli and conditions.