Construction and characterization of a Porphyromonas gingivalis htpG disruption mutant.
Construction and characterization of a Porphyromonas gingivalis htpG disruption mutant.
复制标题
牙龈卟啉单胞菌 htpG 破坏突变体的构建和表征。
DOI：
10.1016/s0378-1097(03)00506-8
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发表时间：
2003
影响因子：
2.1
通讯作者：
Lopatin,DennisE
中科院分区：
文献类型：
--
作者：
Sweier,DomenicaG;Combs,Allison;Shelburne,CharlesE;Fenno,JChristopher;Lopatin,DennisE
我们之前的报道暗示了牙龈卟啉单胞菌（Pg）的Hsp90同源物（HtpG）在牙周病中的毒力。我们研究了HtpG应激蛋白在pg毒力中的作用。本报告描述了(i)重组pghtpg （rHtpG）的表达，（ii）兔抗pgrhtpg抗血清的生成和鉴定，以及（iii） aPg htpGisogenic突变体的构建以及与野生型亲本菌株相比的生长、粘附和侵袭特性的评估。htpggene的破坏对培养的人细胞的生长没有显著影响，对其粘附性和侵袭性也没有影响。
Our previous reports implicated the Hsp90 homologue (HtpG) ofPorphyromonas gingivalis(Pg) in its virulence in periodontal disease. We investigated the role of the HtpG stress protein in the virulence ofPg. This report describes the (i) expression of a recombinantPgHtpG (rHtpG), (ii) generation and characterization of a polyclonal rabbit anti-PgrHtpG antiserum, and (iii) construction of aPg htpGisogenic mutant and evaluation of the growth, adherence and invasion properties compared to the wild-type parental strain. The disruption of thehtpGgene did not significantly affect growth, and had no effect onPgadherence to and invasion of cultured human cells.