The circadian rhythm of salivary cortisol in growing pigs: Effects of age, gender, and stress
The circadian rhythm of salivary cortisol in growing pigs: Effects of age, gender, and stress
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DOI：
10.1016/s0031-9384(97)00177-7
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发表时间：
1997-09-01
影响因子：
2.9
通讯作者：
Koolhaas, JM
中科院分区：
文献类型：
--
作者：
Ruis, MAW;Brake, JHAT;Koolhaas, JM
本实验旨在研究生长猪唾液中皮质醇的昼夜节律性，与年龄，性别和应激（时间）的应用。此外，急性皮质醇反应的压力进行了研究。试验共设5组，每组3头公猪和3头后备母猪。在对照组中，在12、16、20和24周龄时每隔1小时采集唾液样本。在1周内，皮质醇的节律性在两个24小时的跨度（星期一和星期五）进行了评估。用余弦分析评价节律特征，用几个参数描述节律。在12周龄的2组和20周龄的另外2组中，在周四早上或晚上施加压力源（隔离4小时）。同样，在周一和周五通过以2小时间隔采样来评估节律性。急性皮质醇的影响进行了研究，在隔离期间的几个时间点采样。在12至24周龄之间，基础皮质醇浓度下降，在20周龄时达到相当稳定的成人昼夜节律。平均基础皮质醇浓度高于在后备母猪。此外，隔离后，节律的幅度增加，但在公猪母猪不变。节律更不稳定，最大值仅在夜间隔离后才有移动的趋势。应激源的时间，以及年龄，被发现会影响平均皮质醇浓度。此外，应激时间对急性皮质醇反应很重要：早晨的增加更高。本研究的结果强调了在研究动物对应激源的皮质醇反应时，考虑皮质醇的昼夜节律性与年龄、性别和应激源应用（时间）的关系的重要性。(C)1997年爱思唯尔科学公司
This experiment was designed to examine circadian rhythmicity of cortisol in saliva of growing pigs, in relation to age, gender, and (time of) stressor application. Additionally, the acute cortisol response to a stressor was studied. Five groups, each consisting of 3 barrows and 3 gilts, were involved in the experiment. In a Control Group, saliva samples were taken at 1-h intervals at 12, 16, 20, and 24 weeks of age. Within 1 week, rhythmicity of cortisol was assessed during two 24-h spans (Monday and Friday). Rhythm characteristics were evaluated by cosinor analysis, describing the rhythm by several parameters. In 2 groups at 12 weeks and 2 other groups at 20 weeks of age, a stressor was applied (4 h of isolation) on Thursday morning or evening. Again, rhythmicity was assessed on Monday and Friday by sampling at 2-h intervals. Acute cortisol effects were studied by sampling at several time-points during isolation. Between 12 and 24 weeks of age, basal cortisol concentrations decreased and a rather stable and adult circadian rhythm was reached at 20 weeks of age. Average basal cortisol concentrations were higher in barrows than in gilts. Furthermore, after isolation, the amplitude of the rhythm was increased in barrows but was unchanged in gilts. The rhythm was more unstable and the maximum value tended to shift only after evening isolation. Stressor timing, but also age, was found to affect average cortisol concentrations. Moreover, stressor timing was important for the acute cortisol response: the increase was higher in the morning. The results of this study emphasize the importance of considering the circadian rhythmicity of cortisol, in relation to age, gender, and (time of) stressor application, when studying the cortisol response of animals to stressors. (C) 1997 Elsevier Science Inc.