Temporal lobe epilepsy visualized with PET with 11C-L-deuterium-deprenyl -: analysis of kinetic data
Temporal lobe epilepsy visualized with PET with 11C-L-deuterium-deprenyl -: analysis of kinetic data
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DOI：
10.1111/j.1600-0404.1998.tb07300.x
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发表时间：
1998-10-01
影响因子：
3.5
通讯作者：
Langstrom, B
中科院分区：
文献类型：
--
作者：
Bergstrom, M;Kumlien, E;Langstrom, B
目的-本研究的目的是开发一种简化的方法，用于使用配体C-11-L-氘-丙炔基采集和分析正电子发射断层扫描（PET）的数据。这是由于对表征神经退行性疾病和癫痫中神经胶质增生的方法的兴趣增加，其可以由于酶MAO-B的表达增加而被定义。方法：对7例颞叶癫痫患者进行PET检查。在不同的大脑结构中的示踪剂动力学记录和分析使用不同的模型，有和没有血浆输入功能。推导出的值与文献值的H-3-deprenyl结合在冷冻切片从正常人脑。结果-在体内结合和体外数据之间观察到良好的相关性，无论是否使用代谢物校正的血浆或修改的小脑摄取值作为输入函数，相关性都同样良好。癫痫叶，与非癫痫，其特征在于较低的初始分布和增强的后期积累的示踪剂。使用所应用的方法，可以正确识别所有6例单侧患者和1例可能的双侧病例的癫痫侧。结论- PET与C-11-L-氘-丙炔苯丙胺之间的计算体内结合和MAO-B活性的良好相关性。如果使用修改后的小脑时间-活动数据作为参考，则可以简化分析并避免采血。可以生成分布体积和MAO-B结合的单独图像。
Objectives - The purpose of the study was to develop a simplified method for the acquisition and analysis of data from positron emission tomography (PET) using the ligand C-11-L-deuterium-deprenyl. This is motivated by an increased interest in methods to characterize gliosis in neurodegenerative diseases and epilepsy, which can be defined due to an increased expression of the enzyme MAO-B. Methods - Seven patients with temporal lobe epilepsy were investigated with PET. The tracer kinetics in different brain structures was recorded and analyzed using different models with and without a plasma input function. The derived values were correlated to literature values of H-3-deprenyl binding in frozen sections from normal human brains. Results - A good correlation was seen between in vivo binding and in vitro data, with the correlation being equally good irrespective of whether metabolite corrected plasma or modified cerebellar uptake values were used as input function. The epileptic lobe was, compared to non-epileptic, characterized by a lower initial distribution and an enhanced late accumulation of the tracer. With the applied method, it was possible to correctly identify the epileptic side in all 6 unilateral patients and 1 probable bilateral case. Conclusions - PET with C-11-L-deuterium-deprenyl gives a good correlation between calculated in vivo binding and MAO-B activity. The analysis can be simplified and blood sampling avoided if modified cerebellar time-activity data is used as a reference. Separate images of distribution volume and MAO-B binding can be generated.