Bio-distribution and metabolic paths of silica coated CdSeS quantum dots
Bio-distribution and metabolic paths of silica coated CdSeS quantum dots
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二氧化硅包覆的CdSeS量子点的生物分布和代谢途径
DOI：
10.1016/j.taap.2008.03.022
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发表时间：
2008-08-01
影响因子：
3.8
通讯作者：
Fang, Xiaohong
中科院分区：
文献类型：
--
作者：
Chen, Zhen;Chen, Hu;Fang, Xiaohong
随着量子点（QD）技术的快速发展，水溶性量子点有作为特异性诊断的生物探针的前景，但其在体内的生物学行为却鲜为人知。我们最近的体内研究集中于羟基改性二氧化硅网络涂覆的量子点的生物动力学（量子点直径为 21.3 +/- 2.0 nm，最大发射波长为 570 nm）。雄性ICR小鼠静脉注射水溶性QD，单剂量为5 nmol/只。使用电感耦合等离子体质谱法测量 Cd-111 含量，以指示按预定时间间隔收集的血浆、器官和排泄物样品中 QD 的浓度。同时，还通过病理检查和差速离心研究了量子点在组织中的分布和聚集状态。 QD的血浆半衰期和清除率分别为19.8+/-3.2小时和57.3+/-9.2ml/h/kg。肝脏和肾脏是量子点的主要靶器官。量子点根据其体内不同的聚集状态以三种途径代谢。一小部分游离量子点保持其原始形式，可以通过肾小球毛细血管过滤，并在五天内以小分子形式通过尿液排出体外。大多数量子点与蛋白质结合并聚集成较大的颗粒，在肝脏中代谢并通过体内粪便排出体外。五天后，注射剂量的8.6%聚集的QD仍残留在肝组织中，并且这部分很难清除。 (C) 2008 年，爱思唯尔公司出版。
With the rapid development of quantum dot (QD) technology, water-soluble QDs have the prospect of being used as a biological probe for specific diagnoses, but their biological behaviors in vivo are little known. Our recent in vivo studies concentrated on the bio-kinetics of QDs coated by hydroxyl group modified silica networks (the QDs are 21.3 +/- 2.0 nm in diameter and have maximal emission at 570 nm). Male ICR mice were intravenously given the water-soluble QDs with a single dose of 5 nmol/mouse. Inductively coupled plasma-mass spectrometry was used to measure the Cd-111 content to indicate the concentration of QDs in plasma, organs, and excretion samples collected at predetermined time intervals. Meanwhile, the distribution and aggregation state of QDs in tissues were also investigated by pathological examination and differential centrifugation. The plasma half-life and clearance of QDs were 19.8 +/- 3.2 h and 57.3 +/- 9.2 ml/h/kg, respectively. The liver and kidney were the main target organs for QDs. The QDs metabolized in three paths depending on their distinct aggregated states in vivo. A fraction of free QDs, maintaining their original form, could be filtered by glomerular capillaries and excreted via urine as small molecules within five days. Most QDs bound to protein and aggregated into larger particles that were metabolized in the liver and excreted via feces in vivo. After five days, 8.6% of the injected dose of aggregated QDs still remained in hepatic tissue and it was difficult for this fraction to clear. (C) 2008 Published by Elsevier Inc.