Planar Cell Polarity Links Axes of Spatial Dynamics in Neural-Tube Closure
Planar Cell Polarity Links Axes of Spatial Dynamics in Neural-Tube Closure
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DOI：
10.1016/j.cell.2012.04.021
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发表时间：
2012-05-25
期刊：
影响因子：
64.5
通讯作者：
Takeichi, Masatoshi
中科院分区：
文献类型：
--
作者：
Nishimura, Tamako;Honda, Hisao;Takeichi, Masatoshi
神经管闭合是胚胎发育的关键步骤，其失败会导致严重的出生缺陷。两个形态发生过程的协调-会聚延伸和神经板顶端收缩-确保了神经管的完全闭合。我们现在提供的证据表明，平面细胞极性（PCP）信号直接连接这两个过程。在弯曲的神经板，我们发现，一个PCP调节钙粘蛋白，Celsr 1，集中在adherens连接（AJs）面向板的内外侧轴。在这些AJs，Celsr 1与Dishevelled，DAAM 1和PDZ-RhoGEF合作上调Rho激酶，以平面极化方式引起其肌动球蛋白依赖性收缩。这种平面极化收缩促进神经上皮细胞同时的顶端收缩和中线会聚。总之，我们的研究结果表明，PCP信号赋予各向异性收缩的AJs，产生细胞的力量，促进极化弯曲的神经板。
Neural-tube closure is a critical step of embryogenesis, and its failure causes serious birth defects. Coordination of two morphogenetic processes-convergent extension and neural-plate apical constriction-ensures the complete closure of the neural tube. We now provide evidence that planar cell polarity (PCP) signaling directly links these two processes. In the bending neural plates, we find that a PCP-regulating cadherin, Celsr1, is concentrated in adherens junctions (AJs) oriented toward the mediolateral axes of the plates. At these AJs, Celsr1 cooperates with Dishevelled, DAAM1, and the PDZ-RhoGEF to upregulate Rho kinase, causing their actomyosin-dependent contraction in a planar-polarized manner. This planar-polarized contraction promotes simultaneous apical constriction and midline convergence of neuroepithelial cells. Together our findings demonstrate that PCP signals confer anisotropic contractility on the AJs, producing cellular forces that promote the polarized bending of the neural plate.