Characterization of extracellular and substrate-bound cellulases from a mesophilic sugarcane bagasse-degrading microbial community
Characterization of extracellular and substrate-bound cellulases from a mesophilic sugarcane bagasse-degrading microbial community
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DOI：
10.1016/j.procbio.2008.08.001
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发表时间：
2008-12-01
影响因子：
4.4
通讯作者：
Yuan, Hongli
中科院分区：
文献类型：
--
作者：
Lv, Zhiwei;Yang, Jinshui;Yuan, Hongli
甘蔗渣（SCB）是制糖加工的剩余产品，主要含有木质纤维素。纤维素的生物降解是回收SCB 的一种经济方法。为了获得有效的微生物群落并研究SCB生物降解过程，分离了在50℃下生长的SCB降解中温微生物群落EMSD13。超过 77% 的碱预处理 SCB 被降解，超过 83% 的纤维素在 6 天内被 EMSD13 社区利用。为了了解 EMSD13 的生物降解过程，对细胞外纤维素酶和 SCB 可吸收纤维素酶进行了分析。上清液中胞外纤维素酶的峰值活性（42 mU/mL）出现在孵育的第3天。通过用3M盐酸胍洗脱，从残余SCB中回收约73.6mg纤维素酶活性为61.1mU/mg的底物结合蛋白。此外，通过用 6 M 尿素洗脱，从残留的 SCB 中获得了 15.9 mg 的蛋白质，纤维素酶活性为 28.6 mU/mg。 SDS-PAGE 分析表明，两种洗脱液中的纤维素酶相似，但大多数与上清液中的纤维素酶不同。这是关于来自微生物群落的底物结合纤维素酶的第一份报告。 (c) 2008 Elsevier Ltd. 保留所有权利。
Sugarcane bagasse (SCB) is a residual product of sugar processing, containing mainly lignocellulose. The biodegradation of cellulose is an economical approach to recycling SCB. To obtain an effective microbial community and study the SCB biodegradation process, a SCB-degrading mesophilic microbial community, EMSD13, growing at 50 degrees C was isolated. More than 77% of alkali pretreated SCB was degraded and over 83% Of the Cellulose it contained Was utilized by the EMSD13 community within 6 days. To understand the biodegradation procedure of EMSD13, extracellular and SCB-absorbable cellulases were analyzed. The peak activity (42 mU/mL) of the extracellular cellulase in the supernatant Occurred on the 3rd day of incubation. About 73.6 mg of substrate-bound proteins with a cellulase activity of 61.1 mU/mg were recovered from residual SCB by eluting with 3 M guanidine hydrochloride. Also, 15.9 mg of proteins with 28.6 mU/mg of cellulase activity were obtained from residual SCB by eluting with 6 M urea. SDS-PAGE analysis showed that cellulases in both elutes were similar, but most differed from those in the supernatant. This is the first report on substrate-bound cellulases from a microbial community. (c) 2008 Elsevier Ltd. All rights reserved.