Development of a Completely Autologous Valved Conduit With the Sinus of Valsalva Using In-Body Tissue Architecture Technology A Pilot Study in Pulmonary Valve Replacement in a Beagle Model
Development of a Completely Autologous Valved Conduit With the Sinus of Valsalva Using In-Body Tissue Architecture Technology A Pilot Study in Pulmonary Valve Replacement in a Beagle Model
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DOI：
10.1161/circulationaha.109.922211
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发表时间：
2010-09-14
期刊：
影响因子：
37.8
通讯作者：
Nakayama, Yasuhide
中科院分区：
文献类型：
--
作者：
Yamanami, Masashi;Yahata, Yuki;Nakayama, Yasuhide
背景-我们通过简单安全的体内组织构建技术开发了自体假体植入物。我们目前的第一份报告的发展自体瓣膜管道与窦的Valsalva（BIOPHOTOSIDE）通过使用这种独特的技术和随后的植入在肺动脉瓣在beagle model.Methods和结果-A模具的BIOPHOTOSIDE组织组装使用2种类型的专门设计的硅棒与一个小的孔径在三叶形状之间。凹棒有3个突起，类似于主动脉窦的突起。将模具放置在比格犬的背部皮下空间中4周。用自体结缔组织覆盖模具。去除模具后，在带主动脉窦的管道内侧获得了3个瓣叶的生物瓣膜。这些瓣膜具有足够的爆破强度，与自体瓣膜相似。在体外观察到瓣膜紧密接合和足够的开口面积。这些生物瓣膜作为同种异体管道瓣膜植入主肺动脉（n=3）。术后超声心动图显示瓣叶运动平稳，返流轻微。在84天获得的标本的组织学检查表明，小叶的表面覆盖的内皮细胞和新生内膜，包括弹性蛋白纤维网络，并形成在吻合口两侧的管腔表面上的conduine. Conclusion，我们开发了第一个完全自体的BIOVALVE和成功地植入这些BIOVALVE在小猎犬模型中的试点研究。（循环。2010;122[suppl 1]：S100-S106.）
Background-We developed autologous prosthetic implants by simple and safe in-body tissue architecture technology. We present the first report on the development of autologous valved conduit with the sinus of Valsalva (BIOVALVE) by using this unique technology and its subsequent implantation in the pulmonary valves in a beagle model.Methods and Results-A mold of BIOVALVE organization was assembled using 2 types of specially designed silicone rods with a small aperture in a trileaflet shape between them. The concave rods had 3 projections that resembled the protrusions of the sinus of Valsalva. The molds were placed in the dorsal subcutaneous spaces of beagle dogs for 4 weeks. The molds were covered with autologous connective tissues. BIOVALVEs with 3 leaflets in the inner side of the conduit with the sinus of Valsalva were obtained after removing the molds. These valves had adequate burst strength, similar to that of native valves. Tight valvular coaptation and sufficient open orifice area were observed in vitro. These BIOVALVEs were implanted to the main pulmonary arteries as allogenic conduit valves (n=3). Postoperative echocardiography demonstrated smooth movement of the leaflets with trivial regurgitation. Histological examination of specimens obtained at 84 days showed that the surface of the leaflet was covered by endothelial cells and neointima, including an elastin fiber network, and was formed at the anastomosis sides on the luminal surface of the conduit.Conclusion-We developed the first completely autologous BIOVALVE and successfully implanted these BIOVALVEs in a beagle model in a pilot study. (Circulation. 2010;122[suppl 1]:S100-S106.)