On tight junction structure: Forssman glycolipid does not flow between MDCK cells in an intact epithelial monolayer.
On tight junction structure: Forssman glycolipid does not flow between MDCK cells in an intact epithelial monolayer.
复制标题
关于紧密连接结构：福斯曼糖脂不会在完整的上皮单层中的 MDCK 细胞之间流动。
DOI：
10.1016/s0006-291x(86)80404-1
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发表时间：
1986
影响因子：
3.1
通讯作者：
YoungJr,WW
中科院分区：
文献类型：
--
作者：
Nichols,GE;Borgman,CA;YoungJr,WW
One model of tight junction structure suggests that lipids might flow from cell to cell within shared exoplasmic membrane leaflets. We tested this proposal by co-culturing two clones of MDCK epithelial cells, which differed in their content of Forssman glycolipid, and then staining by immunofluorescence with rabbit anti-Forssman Ig. In co-cultures grown on glass cover slips and on nitrocellulose filters, positive Forssman staining was restricted to sharply demarcated clusters of cells formed by the Forssman-positive clone. Integrity of tight junctions between the two clones was indicated on cover slips by the presence of individual domes (hemicysts) composed of both clones and on filters by the generation of transepithelial potential differences. These results suggest that glycolipids in the exoplasmic leaflet of cells in a tight epithelium do not flow to adjacent cells.
