Heat treatment of curdlan enhances the enzymatic production of biologically active β-(1,3)-glucan oligosaccharides
Heat treatment of curdlan enhances the enzymatic production of biologically active β-(1,3)-glucan oligosaccharides
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DOI：
10.1016/j.carbpol.2016.03.066
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发表时间：
2016-08-01
影响因子：
11.2
通讯作者：
Kimura, Atsuo
中科院分区：
文献类型：
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作者：
Kumagai, Yuya;Okuyama, Masayuki;Kimura, Atsuo
利用GH64酶(KfGH64)从柯德兰中制备生物活性β-(1，3)-葡聚糖低聚糖。KfGH64对天然凝胶酶活性较低，因此对凝胶酶的前处理条件进行了评价。KfGH64在热处理条件下对可德聚糖的催化活性最高。在优化的酶反应条件下(pH 5.5，温度37℃，搅拌速度100rpm，反应时间24 h，KfGH64用量为10mgKfGH64/1g)，最有效的预处理(90℃，0.5h)可将60%左右的凝胶糖转化为可溶性糖。所得产物主要为层状五糖和少量的p-(1，3)-葡聚糖，平均聚合度(DP)分别为13和130。产物中不含小分子低聚糖(dp<；5)，说明KfGH64水解法是制备生物活性β-(1，3)-葡聚糖的合适方法。(C)2016爱思唯尔有限公司。保留所有权利。
Biologically active beta-(1,3)-glucan oligosaccharides were prepared from curdlan using GH64 enzyme (KfGH64). KfGH64 showed low activity toward native curdlan; thereby pretreatment conditions of curdlan were evaluated. KfGH64 showed the highest activity toward curdlan with heat treatment. The most efficient pretreatment (90 degrees C for 0.5 h) converted approximately 60% of curdlan into soluble saccharides under the optimized enzyme reaction conditions (pH 5.5, 37 degrees C, 100 rpm mixing speed, 24 h, and 10 mu g of KfGH64/1 g of curdlan). The resulting products were predominantly laminaripentaose and a small amount of p-(1,3)-glucans with an average degree of polymerization (DP) of 13 and 130. The products did not contain small oligosaccharides (DP< 5), indicating that the hydrolysis of heat-treated curdlan by KfGH64 is a suitable method for the production of biologically active beta-(1,3)-glucan oligosaccharides. (C) 2016 Elsevier Ltd. All rights reserved.