An improved method for the isolation of hexuronic acid from chondroitin sulphate preparations.
An improved method for the isolation of hexuronic acid from chondroitin sulphate preparations.
复制标题
从硫酸软骨素制剂中分离己糖醛酸的改进方法。
DOI：
10.1016/0304-4165(67)90258-9
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发表时间：
1967
期刊：
影响因子：
--
通讯作者：
K. G. Rienits
中科院分区：
文献类型：
--
作者：
P. Jeffrey;K. G. Rienits
硫酸软骨素生物合成的最新研究强调了需要一种方法，以可观的产率分离组分单糖，用于随后的分析等。特别是己糖醛酸，因为它们在释放其他单糖所需的强水解条件下被广泛破坏，因此造成了问题1。酶解是不可行的2。在水解之前用硼氢化物还原糖醛酸残基的羧基产生多糖材料，从该多糖材料可以高产率获得源自糖醛酸的己糖3，4。然而，在使用葡萄糖作为前体的生物合成研究中，将糖酸转化为己糖可能会引起并发症，因为硫酸软骨素和软骨粘蛋白S-L的样品中存在己糖（主要是半乳糖）。此外，少量高比活性的前体葡萄糖可能是污染物。用磺化聚苯乙烯树脂在稀酸（例如0.05 M HCl）中实现了温和但有效的水解条件（参考文献104）。8-1o）。Anastassiadis和COMMON 10-1特别利用该技术从动物组织中水解含碳水化合物的物质。从剩余的水解产物中分离出含己糖胺的级分，但没有尝试进一步检查或纯化级分。
Recent interest in the biosynthesis of the chondroitin sulphates has emphasized the need for a procedure permitting the isolation of the constituent monosaccharides in appreciable yields for subsequent analyses etc. The hexuronic acids in particular pose problems since they are extensively destroyed during the strong hydrolysis conditions necessary to release other monosaccharides 1. Enzymic hydrolysis is not practicable 2. Reduction with borohydride of the carboxyl group of the uronic acid residue prior to hydrolysis produces polysaccharide material from which hexose derived from the uronic acid can be obtained in high yield3, 4. However, the transformation of nronic acid to hexose could introduce complications in biosynthetic investigations where [z4Clglucose was being used as a precursor since hexose (mainly galactose) is present in samples of chondroitin sulphate and chondromucoproteinS-L Also small quantities of precursor glucose of high specific activity could be a contaminant. Mild but effective conditions for hydrolysis have been achieved with sulphonated polystyrene resin in dilute acid, eg 0.05 M HC1 (refs. 8-1o). ANASTASSIADIS AND COMMON 10-1~ in particular utilized this technique to hydrolyse carbohydrate-containing materials from animal tissues. A hexosamine-containing fraction was separated from the remainder of the hydrolysis products but no further examination or purification of the fractions was attempted.