Self‐Assembled Micro‐Sized Hexagons Built from Short DNA in a Crowded Environment
Self‐Assembled Micro‐Sized Hexagons Built from Short DNA in a Crowded Environment
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在拥挤环境中用短 DNA 自组装微型六边形
DOI：
10.1002/cbic.202200360
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发表时间：
2022
期刊：
影响因子：
3.2
通讯作者：
Tanaka Makiko
中科院分区：
文献类型：
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作者：
Makino Tetsunao;Nakane Daisuke;Tanaka Makiko
各种形态的DNA可编程结构因其在材料科学和生物医学中的潜在应用而引起了广泛的关注。在此，我们报道了在聚乙二醇缓冲盐溶液中，通过一对短双链DNA的组装，形成了微米级的六角形。每条DNA链都有互补的碱基，带有两个碱基的突出部分。钝端双链DNA的加热和随后的冷却过程导致了不同的组装。这些结果表明，构建六角形DNA组装需要互补悬挑诱导的末端端到端的粘连。六边形的稳定形成高度依赖于加热温度。此外，DNA和聚乙二醇组分的浓度调节也是必不可少的。圆二色谱测量和偏光显微镜观察表明，六角形血小板中的双链DNA平行排列。由简单的积木组成的自组装微尺寸六边形可能在未来的生物医学设备开发中具有巨大的潜力。
DNA programmable structures of various morphologies have attracted extensive attention due to their potential for materials science and biomedical applications. Here, we report the formation of micro‐sized hexagonsviaassembly of only one pair of short double‐stranded DNA in buffer‐salt poly(ethylene glycol) solution. Each DNA strand had complementary bases with a two‐base overhang. The procedure of heating and subsequent cooling of blunt‐ended double‐stranded DNA resulted in different assemblies. These results indicated that end‐to‐end adhesion at the terminals induced by complementary overhangs were required to construct the hexagonal DNA assemblies. The stable formation of the hexagons was highly dependent on heating temperature. In addition, concentration adjustments of DNA and poly(ethylene glycol) were essential. Circular dichroism spectral measurements and polarization microscopy observations indicated parallel alignment of double‐stranded DNA in the hexagonal platelet. Self‐assembled micro‐sized hexagons composed of simple building blocks may have great potential for future biomedical device development.