Biological assessment (antiviral and antioxidant) and acute toxicity of essential oils from Drimys angustifolia and D. brasiliensis
Biological assessment (antiviral and antioxidant) and acute toxicity of essential oils from Drimys angustifolia and D. brasiliensis
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狭叶鳢和巴西鳢精油的生物学评估（抗病毒和抗氧化）和急性毒性
DOI：
10.1590/s0102-695x2012005000142
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发表时间：
2013
影响因子：
1.6
通讯作者：
R. Limberger
中科院分区：
文献类型：
--
作者：
Madson R F Gomes;R. Schuh;A. L. Jacques;G. G. Dorneles;J. Montanha;P. Roehe;Sérgio A L Bordignon;E. Dallegrave;M. Leal;R. Limberger
冬虫夏草属是冬虫夏科植物中地理分布最广的一种，共有7属约120种。在巴西，发现该属从巴伊亚到南里奥格兰德州，并出现在两个种，Drimys angustifolia Miers和D.brasiliens Miers，冬季科，俗称“瀑布-安塔”，其特征是存在香叶类化合物和精油。在民间医学中，除具有杀虫作用外，还可用作抗坏血酸、兴奋剂、解痉剂、止泻、解热、抗菌、治疗哮喘和支气管炎。除了已知的生物活性外，在治疗寄生虫引起的生理疾病或疾病方面探索新的应用是非常重要的。基于这一信息，在本研究中，我们建议使用DPPH自由基抗人类疱疹病毒1型(HSV-1)的模型和体内急性毒性来评价巴西杜鹃和狭叶杜鹃的挥发油作为抗氧化剂。这两个物种不能降低DPPH自由基，并显示出有趣的抗病毒活性，在体外试验中显著降低了病毒滴度。关于雌性Wistar大鼠的体内毒性，这两种药物在动物身上显示出有趣的症状，如流口水、上睑下垂、震颤、运动能力下降。除巴西石杉的油等体征外，部分动物表现出排尿增多和腹泻。
The genus Drimys presents the widest geographical distribution of the Winteraceae family, which comprises seven genera and about 120 species. In Brazil, the genus is found from Bahia to Rio Grande do Sul and occur in two species, Drimys angustifolia Miers, and D. brasiliensis Miers, Winteraceae, popularly known as “cascade- anta”, characterized by the presence of fl avonoids and essential oils. It is used in folk medicine as an antiscorbutic, stimulant, antispasmodic, anti-diarrheal, antipyretic, antibacterial, and against asthma and bronchitis, besides having insecticidal properties. In addition to the known biological activities, it is very important to explore new applications in the treatment of physiological disorders or diseases caused by parasites. Based on this information, in this study we propose to evaluate volatile oils of the species D. brasiliensis and D. angustifolia, as an antioxidant, using the model of the DPPH radical as an antiviral against human herpes virus type 1 (HSV-1) and acute toxicity in vivo. The two species were not able to reduce the DPPH radical and showed interesting antiviral activity, signifi cantly reducing the virus titers in vitro assays. Regarding the in vivo toxicity in female Wistar rats, treatment with the two species showed interesting signs in animals such as salivation, ptosis, tremor, decreased motor activity. In addition the oils of D. brasiliensis to other signs, some animals showed increased urination and diarrhea.