Quantitative phase imaging of arthropods.
Quantitative phase imaging of arthropods.
复制标题
节肢动物的定量相位成像。
DOI：
10.1117/1.jbo.20.11.111212
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发表时间：
2015
影响因子：
3.5
通讯作者：
Popescu,Gabriel
中科院分区：
文献类型：
--
作者：
Sridharan,Shamira;Katz,Aron;Soto-Adames,Felipe;Popescu,Gabriel
节肢动物的分类是通过描述细小的特征，如刚毛和角质层来完成的。安装在玻片上的未染色的完整节肢动物标本可以保存数十年，但由于目前的方法需要进行样本处理或繁琐的图像处理，因此很难进行研究。空间光干涉显微镜(SLIM)是一种定量相位成像(QPI)技术，是商用相差显微镜的附加模块。我们使用SLIM在一张幻灯片上描绘了一个完整的有机体。据我们所知，这是第一次使用QPI对整个生物体进行成像。我们还展示了SLIM成像精细结构的能力，此外还提供了传统的明场显微镜无法获得的定量数据。
Classification of arthropods is performed by characterization of fine features such as setae and cuticles. An unstained whole arthropod specimen mounted on a slide can be preserved for many decades, but is difficult to study since current methods require sample manipulation or tedious image processing. Spatial light interference microscopy (SLIM) is a quantitative phase imaging (QPI) technique that is an add-on module to a commercial phase contrast microscope. We use SLIM to image a whole organism springtailCeratophysella denticulatamounted on a slide. This is the first time, to our knowledge, that an entire organism has been imaged using QPI. We also demonstrate the ability of SLIM to image fine structures in addition to providing quantitative data that cannot be obtained by traditional bright field microscopy.