Design Mechanism and Property of the Novel Fluorescent Probes for the Identification of Microthrix Parvicella In Situ.
Design Mechanism and Property of the Novel Fluorescent Probes for the Identification of Microthrix Parvicella In Situ.
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DOI：
10.3390/ma10070804
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发表时间：
2017-07-15
期刊：
影响因子：
--
通讯作者：
Zhang B
中科院分区：
文献类型：
--
作者：
Jiao X;Fei X;Li S;Lin D;Ma H;Zhang B
本研究以微小微丝菌（Microthrix parvicella，M. parvicella），更喜欢利用长链脂肪酸（LCFA）标记M.活性污泥中的细小杆菌探针A和探针B的分子结构分别为长链烷烃和LCFA。结果表明，探针A和探针B的Stokes位移分别为118 nm和120 nm，量子产率分别为0.1043和0.1058，有利于荧光标记。由于探针A在活性污泥中的稳定性优于探针B，且在24 h内荧光强度保持稳定，因此探针A更适合标记M.原位的小囊菌。此外，通过Image Pro Plus 6（IPP 6）分析，建立了标记M的污泥体积指数（SVI）与积分光密度（IOD）之间的定量关系。活性污泥样品中的细小杆菌。IOD与SVI的关系符合Logistic曲线（R2 = 0.94）。
In this study, two novel fluorescent probes, probe A and probe B were designed, synthesized and characterized, based on Microthrix parvicella (M. parvicella) preferring to utilize long-chain fatty acid (LCFA), for the labeling of M. parvicella in activated sludge. The molecular structure of probe A and probe B include long-chain alkane and LCFA, respectively. The results indicated that probe A and probe B had a large stokes shift of 118 nm and 120 nm and high quantum yield of 0.1043 and 0.1058, respectively, which were significantly helpful for the fluorescent labeling. As probe A was more stable than probe B in activated sludge, and the fluorescence intensity keep stable during 24 h, probe A was more suitable for labeling M. parvicella in situ. In addition, through the Image Pro Plus 6 (IPP 6) analysis, a quantitative relationship was established between sludge volume index (SVI) and integral optical density (IOD) of the labeled M. parvicella in activated sludge samples. The relationship between IOD and SVI conforms to Logistic curve (R2 = 0.94).
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