Graphene-like nanoribbons periodically embedded with four- and eight-membered rings
Graphene-like nanoribbons periodically embedded with four- and eight-membered rings
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通过相关成像实时可视化金纳米粒子的聚类和细胞内运输
DOI：
10.1038/ncomms15646
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发表时间：
2017-03-31
影响因子：
16.6
通讯作者：
Zhong, Dingyong
中科院分区：
文献类型：
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作者：
Liu, Meizhuang;Liu, Mengxi;Zhong, Dingyong
从机理上了解纳米颗粒的内吞作用和细胞内运输对于设计智能治疗载体是至关重要的。纳米颗粒的物理化学性质，包括尺寸、聚集和表面化学，调节着它们在细胞中的摄取和运输。值得注意的是，即使是单一的纳米颗粒也可以聚集在细胞内，但它们的聚集状态和随后的贩运还没有被很好地了解。在这里，我们使用DNA修饰的金(fPlas-金)纳米颗粒作为双发射荧光和等离子体探针来检测它们的聚集状态和细胞内运输。相关的荧光和等离子成像证据表明，fPlas-Gold的内吞作用遵循多种途径。在内吞作用的早期，fPlas-金纳米颗粒大多以单颗粒形式出现，在囊泡运输和成熟过程中聚集在一起。包裹fPlas-Gold的运输速度严重依赖于细胞团的大小，而不是细胞器的类型，如内小体和溶酶体。我们的结果为设计治疗纳米载体以实现有效的健康管理提供了关键策略。
Mechanistic understanding of the endocytosis and intracellular trafficking of nanoparticles is essential for designing smart theranostic carriers. Physico-chemical properties, including size, clustering and surface chemistry of nanoparticles regulate their cellular uptake and transport. Significantly, even single nanoparticles could cluster intracellularly, yet their clustering state and subsequent trafficking are not well understood. Here, we used DNA-decorated gold (fPlas-gold) nanoparticles as a dually emissive fluorescent and plasmonic probe to examine their clustering states and intracellular transport. Evidence from correlative fluorescence and plasmonic imaging shows that endocytosis of fPlas-gold follows multiple pathways. In the early stages of endocytosis, fPlas-gold nanoparticles appear mostly as single particles and they cluster during the vesicular transport and maturation. The speed of encapsulated fPlas-gold transport was critically dependent on the size of clusters but not on the types of organelle such as endosomes and lysosomes. Our results provide key strategies for engineering theranostic nanocarriers for efficient health management.