Biochemical interactions between proteins and mat1 cis-acting sequences required for imprinting in fission yeast.
Biochemical interactions between proteins and mat1 cis-acting sequences required for imprinting in fission yeast.
复制标题
裂殖酵母中印记所需的蛋白质和 mat1 顺式作用序列之间的生化相互作用。
DOI：
10.1128/mcb.24.22.9813-9822.2004
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发表时间：
2004
影响因子：
5.3
通讯作者：
Klar,AmarJS
中科院分区：
文献类型：
--
作者：
Lee,Bum-Soo;Grewal,ShivIS;Klar,AmarJS
DNA recombination required for mating type (mat1) switching inSchizosaccharomyces pombeis initiated bymat1imprinting. The imprinting event is regulated bymat1 cis-acting elements and by severaltrans-acting factors, includingswi1(for switch),swi3,swi7, andsap1. swi1andswi3were previously shown to function in dictating unidirectionalmat1DNA replication by controlling replication fork movement around themat1region and, second, by pausing fork progression around the imprint site. With biochemical studies, we investigated whether thetrans-acting factors function indirectly or directly by binding to themat1 cis-acting sequences. First, we report the identification and DNA sequence of theswi3gene.swi3is not essential for viability, and, like the other factors, it exerts a stimulatory effect on imprinting. Second, we showed that only Swi1p and Swi3p interact to form a multiprotein complex and that complex formation did not require their binding to a DNA region defined by thesmt-0mutation. Third, we found that the Swi1p-Swi3p complex physically binds to a region around the imprint site where pausing of replication occurs. Fourth, the protein complex also interacted with themat1-proximal polar terminator of replication (RTS1). These results suggest that the stimulatory effect ofswi1andswi3on switching and imprinting occurs through interaction of the Swi1p-Swi3p complex with themat1regions.
