Copper Oxide Nanoparticle Mediated DNA Damage in Terrestrial Plant Models
Copper Oxide Nanoparticle Mediated DNA Damage in Terrestrial Plant Models
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DOI：
10.1021/es202660k
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发表时间：
2012-02-07
影响因子：
11.4
通讯作者：
Nelson, Bryant C.
中科院分区：
文献类型：
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作者：
Atha, Donald H.;Wang, Huanhua;Nelson, Bryant C.
工程纳米粒子由于其独特的电学、机械和催化性能，目前在许多商业产品中都有存在，并将随着浓度的增加而有意或无意地释放到自然环境中。基于金属和金属氧化物的纳米材料已被证明是哺乳动物细胞、生物体甚至细菌中DNA损伤的媒介，但这种损伤发生的分子机制尚不清楚。我们首次报道了纳米氧化铜对农业和草原植物的DNA损伤。在控制的实验室条件下，萝卜、多年生黑麦草和一年生黑麦草的DNA氧化损伤(7，8-二氢-8-氧鸟嘌呤；2，6-二氨基-4-羟基-5-甲酰胺基嘧啶；4，6-二氨基-5-甲酰胺基嘧啶)显著积累，并对植物生长有很强的抑制作用。同时测定了铜离子和大尺度铜粒介导的损伤累积水平，以阐明DNA损伤的机制。据我们所知，这是植物中多个DNA损伤形成和积累的第一个证据。这些发现为未来研究纳米颗粒介导的植物DNA损伤和修复机制提供了动力。
Engineered nanopartides, due to their unique electrical, mechanical, and catalytic properties, are presently found in many commercial products and will be intentionally or inadvertently released at increasing concentrations into the natural environment. Metal- and metal oxide-based nanomaterials have been shown to act as mediators of DNA damage in mammalian cells, organisms, and even in bacteria, but the molecular mechanisms through which this occurs are poorly understood. For the first time, we report that copper oxide nanopartides induce DNA damage in agricultural and grassland plants. Significant accumulation of oxidatively modified, mutagenic DNA lesions (7,8-dihydro-8-oxoguanine; 2,6-diamino-4-hydroxy-5-formamidopyrimidine; 4,6-diamino-5-formamidopyrimidine) and strong plant growth inhibition were observed for radish (Raphanus sativus), perennial ryegrass (Lolium perenne), and annual ryegrass (Lolium rigidum) under controlled laboratory conditions. Lesion accumulation levels mediated by copper ions and macroscale copper particles were measured in tandem to clarify the mechanisms of DNA damage. To our knowledge, this is the first evidence of multiple DNA lesion formation and accumulation in plants. These findings provide impetus for future investigations on nanoparticle-mediated DNA damage and repair mechanisms in plants.