Characterization of plasma membrane H+‐ATPase from salt‐tolerant yeast Candida versatilis
Characterization of plasma membrane H+‐ATPase from salt‐tolerant yeast Candida versatilis
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DOI：
10.1002/yea.320090302
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发表时间：
1993-03
期刊：
影响因子：
2.6
通讯作者：
Yasuo Watanabe;M. Yamaguchi;Jun Sakamoto;Y. Tamai
中科院分区：
文献类型：
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作者：
Yasuo Watanabe;M. Yamaguchi;Jun Sakamoto;Y. Tamai
从耐盐酵母假丝酵母中分离质膜，并表征质膜中的ATP酶。ATP酶是典型的H+-ATP酶，具有与酿酒酵母和鲁氏接合酵母酶相似的性质。它与抗S.酿酒酵母质膜H+-ATP酶。NaCl和KCl对ATP酶活性无明显影响，但NH 4+可使ATP酶活性升高，硫酸铵的作用尤为明显。在体内，通过向培养基中加入NaCl观察到ATP酶活性的刺激，如在Z. rouxii。在C中未观察到葡萄糖代谢对H+-ATP酶的体内激活。与Z. rouxii和S.酿酒酵母细胞
Plasma membrane was isolated from the salt‐tolerant yeast Candida versatilis and the ATPase in plasma membrane was characterized. The ATPase was a typical H+‐ATPase with similar properties to the Saccharomyces cerevisiae and Zygosaccharomyces rouxii enzymes. It was reacted with antibody (IgG) raised against S. cerevisiae plasma membrane H+‐ATPase. The ATPase activity was not changed by adding NaCl and KCl to the assay solutions, but was increased by NH 4+ , especially by ammonium sulfate. In vivo stimulation of ATPase activity was observed by the addition of NaCl into the culture medium, as observed in Z. rouxii. No in vivo activation of H+‐ATPase by glucose metabolism was observed in C. versatilis cells and the activity was independent of the growth phase, like Z. rouxii and unlike S. cerevisiae cells.